Abstract
RE-1 silencing transcription factor (REST), a master negative regulator of neuronal differentiation, controls neurogenesis by preventing the differentiation of neural stem cells. Here we focused on the role of REST in the early steps of differentiation and maturation of adult hippocampal progenitors (AHPs). REST knockdown promoted differentiation and affected the maturation of rat AHPs. Surprisingly, REST knockdown cells enhanced the differentiation of neighboring wild-type AHPs, suggesting that REST may play a non-cellautonomous role. Gene expression analysis identified Secretogranin II (Scg2) as the major secreted REST target responsible for the non-cell-autonomous phenotype. Loss-of-function of Scg2 inhibited differentiation in vitro, and exogenous SCG2 partially rescued this phenotype. Knockdown of REST in neural progenitors in mice led to precocious maturation into neurons at the expense of mushroom spines in vivo. In summary, we found that, in addition to its cell-autonomous function, REST regulates differentiation and maturation of AHPs non-cell-autonomously via SCG2.
Original language | English (US) |
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Pages (from-to) | 14872-14884 |
Number of pages | 13 |
Journal | Journal of Neuroscience |
Volume | 35 |
Issue number | 44 |
DOIs | |
State | Published - Nov 4 2015 |
Externally published | Yes |
Keywords
- Adult neurogenesis
- Microfludics
- Neurosecretory
- REST/NRSF
- SCG2
- Stem cells
ASJC Scopus subject areas
- General Neuroscience