Restoration of the wild-type phenotype in Pichia guilliermondii transformants

Yu V. Pinyaga, T. M. Prokopiv, A. V. Petrishin, O. V. Khalimonchuk, O. V. Protchenko, D. V. Fedorovich, Yu R. Boretsky

Research output: Contribution to journalArticle

Abstract

Pichia guilliermondii strain with blocked GTP cyclohydrolase II was transformed using replicative plasmids and their fragments containing the structural gene RIB1 of this enzyme. Experiments showed that the presence of the ARS element and the promoter region of this gene in the genome of transformants reduces the probability of their reversion to the wild-type phenotype. Different types of recombination in the yeast P. guilliermondii are discussed.

Original languageEnglish (US)
Pages (from-to)314-318
Number of pages5
JournalMicrobiology
Volume71
Issue number3
DOIs
StatePublished - May 1 2002

Keywords

  • GTP cyclohydrolase II
  • Recombination types
  • Riboflavin biosynthesis
  • Transformation
  • Yeast Pichia guilliermondii

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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    Pinyaga, Y. V., Prokopiv, T. M., Petrishin, A. V., Khalimonchuk, O. V., Protchenko, O. V., Fedorovich, D. V., & Boretsky, Y. R. (2002). Restoration of the wild-type phenotype in Pichia guilliermondii transformants. Microbiology, 71(3), 314-318. https://doi.org/10.1023/A:1015858712461