Restoration of the wild-type phenotype in Pichia guilliermondii transformants

Yu V. Pinyaga; T. M. Prokopiv; A. V. Petrishin; O. V. Khalimonchuk; O. V. Protchenko; D. V. Fedorovich; Yu R. Boretsky

doi:10.1023/A:1015858712461

Restoration of the wild-type phenotype in Pichia guilliermondii transformants

Yu V. Pinyaga, T. M. Prokopiv, A. V. Petrishin, O. V. Khalimonchuk, O. V. Protchenko, D. V. Fedorovich, Yu R. Boretsky

Research output: Contribution to journal › Article › peer-review

Abstract

Pichia guilliermondii strain with blocked GTP cyclohydrolase II was transformed using replicative plasmids and their fragments containing the structural gene RIB1 of this enzyme. Experiments showed that the presence of the ARS element and the promoter region of this gene in the genome of transformants reduces the probability of their reversion to the wild-type phenotype. Different types of recombination in the yeast P. guilliermondii are discussed.

Original language	English (US)
Pages (from-to)	314-318
Number of pages	5
Journal	Microbiology
Volume	71
Issue number	3
DOIs	https://doi.org/10.1023/A:1015858712461
State	Published - May 2002
Externally published	Yes

Keywords

GTP cyclohydrolase II
Recombination types
Riboflavin biosynthesis
Transformation
Yeast Pichia guilliermondii

ASJC Scopus subject areas

Microbiology
Applied Microbiology and Biotechnology

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title = "Restoration of the wild-type phenotype in Pichia guilliermondii transformants",

abstract = "Pichia guilliermondii strain with blocked GTP cyclohydrolase II was transformed using replicative plasmids and their fragments containing the structural gene RIB1 of this enzyme. Experiments showed that the presence of the ARS element and the promoter region of this gene in the genome of transformants reduces the probability of their reversion to the wild-type phenotype. Different types of recombination in the yeast P. guilliermondii are discussed.",

keywords = "GTP cyclohydrolase II, Recombination types, Riboflavin biosynthesis, Transformation, Yeast Pichia guilliermondii",

author = "Pinyaga, {Yu V.} and Prokopiv, {T. M.} and Petrishin, {A. V.} and Khalimonchuk, {O. V.} and Protchenko, {O. V.} and Fedorovich, {D. V.} and Boretsky, {Yu R.}",

year = "2002",

month = may,

doi = "10.1023/A:1015858712461",

language = "English (US)",

volume = "71",

pages = "314--318",

journal = "Microbiology",

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T1 - Restoration of the wild-type phenotype in Pichia guilliermondii transformants

AU - Pinyaga, Yu V.

AU - Prokopiv, T. M.

AU - Petrishin, A. V.

AU - Khalimonchuk, O. V.

AU - Protchenko, O. V.

AU - Fedorovich, D. V.

AU - Boretsky, Yu R.

PY - 2002/5

Y1 - 2002/5

N2 - Pichia guilliermondii strain with blocked GTP cyclohydrolase II was transformed using replicative plasmids and their fragments containing the structural gene RIB1 of this enzyme. Experiments showed that the presence of the ARS element and the promoter region of this gene in the genome of transformants reduces the probability of their reversion to the wild-type phenotype. Different types of recombination in the yeast P. guilliermondii are discussed.

AB - Pichia guilliermondii strain with blocked GTP cyclohydrolase II was transformed using replicative plasmids and their fragments containing the structural gene RIB1 of this enzyme. Experiments showed that the presence of the ARS element and the promoter region of this gene in the genome of transformants reduces the probability of their reversion to the wild-type phenotype. Different types of recombination in the yeast P. guilliermondii are discussed.

KW - GTP cyclohydrolase II

KW - Recombination types

KW - Riboflavin biosynthesis

KW - Transformation

KW - Yeast Pichia guilliermondii

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DO - 10.1023/A:1015858712461

M3 - Article

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VL - 71

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EP - 318

JO - Microbiology

JF - Microbiology

IS - 3

ER -

Restoration of the wild-type phenotype in Pichia guilliermondii transformants

Abstract

Keywords

ASJC Scopus subject areas

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