Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga

Yunnan Xia; Dwight E. Burbank; James L. van Etten

doi:10.1093/nar/14.15.6017

Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga

Yunnan Xia, Dwight E. Burbank, James L. van Etten

Research output: Contribution to journal › Article › peer-review

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Abstract

A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.

Original language	English (US)
Pages (from-to)	6017-6030
Number of pages	14
Journal	Nucleic acids research
Volume	14
Issue number	15
DOIs	https://doi.org/10.1093/nar/14.15.6017
State	Published - Aug 11 1986

ASJC Scopus subject areas

Genetics

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title = "Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga",

abstract = "A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.",

author = "Yunnan Xia and Burbank, {Dwight E.} and {van Etten}, {James L.}",

note = "Funding Information: helpful discussions with Myron Brakke, Les Lane, and Russ Meints are gratefully acknowledged. This investigation was supported, in part, by Public Health Service grant GM-32441 from the National Institute of General Medical Sciences and grant DE-ACO2-82ER12086 from the Department of Energy.",

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doi = "10.1093/nar/14.15.6017",

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T1 - Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga

AU - Xia, Yunnan

AU - Burbank, Dwight E.

AU - van Etten, James L.

N1 - Funding Information: helpful discussions with Myron Brakke, Les Lane, and Russ Meints are gratefully acknowledged. This investigation was supported, in part, by Public Health Service grant GM-32441 from the National Institute of General Medical Sciences and grant DE-ACO2-82ER12086 from the Department of Energy.

PY - 1986/8/11

Y1 - 1986/8/11

N2 - A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.

AB - A type II restriction endonuclease, CviBI, was isolated from a eukaryotic, Chlorella-like green alga infected with the dsDNA containing virus NC-1A. The enzyme recognizes the sequence GANTC and cleaves DNA between the G and A. Methylation of deoxyadenosine in the GANTC sequence probably inhibits enzyme activity. In vitro CviBI cleaves host nuclear DNA but not viral DNA. A survey of 18 other viruses which infect the same Chlorella sp. revealed that infection with 5 of these viruses also induced a restriction endonuclease which cleaves DNA into the same size fragments as CviBI.

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Restriction endonuclease activity induced by NC-1A virus infection of a Chlorella-like green alga

Abstract

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