Restriction of HIV Replication in Infected T Cells and Monocytes by Interferon-α

Howard E. Gendelman, Lisa Baca, Jim A. Turpin, D. Chester Kalter, Brian D. Hansen, Jan M. Orenstein, Robert M. Friedman, Monte S. Meltzer

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

Human recombinant interferon-α (IFNα) restricted viral replication in human immunodeficiency virus- (HIV) infected T cells and monocytes. With T cells, reverse transcriptase (RT) activity in culture fluids was reduced threefold from that of control infected cells by IFN treatment, but HIV p24 antigen levels were unchanged. In contrast, levels of p24 antigen and RT activity in lysates of IFN-treated infected cells were threefold greater than those of controls. These differences suggest that the mechanism for IFN-induced antiviral effects in HIV-infected T cells resides in the terminal events (assembly and release) of the virus replication cycle. Monocytes treated with IFN at the time of virus challenge showed no p24 antigen or RT activity, no HIV-specific mRNA, and no proviral DNA in cells for up to 3 weeks after infection. IFN treatment of chronically infected monocytes also decreased virus replication, as assessed by p24 antigen, mRNA and RT detection assays. However, levels of proviral DNA in the IFN-treated and control HIV-infected cells were indistinguishable. The presence of large quantities of proviral DNA in cells with little or no evidence for active transcription documents a situation approaching true microbiological latency.

Original languageEnglish (US)
Pages (from-to)1045-1049
Number of pages5
JournalAIDS Research and Human Retroviruses
Volume6
Issue number8
DOIs
StatePublished - Aug 1990

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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    Gendelman, H. E., Baca, L., Turpin, J. A., Kalter, D. C., Hansen, B. D., Orenstein, J. M., Friedman, R. M., & Meltzer, M. S. (1990). Restriction of HIV Replication in Infected T Cells and Monocytes by Interferon-α. AIDS Research and Human Retroviruses, 6(8), 1045-1049. https://doi.org/10.1089/aid.1990.6.1045