Retinoic acid is a negative regulator of the neuropeptide Y/ peptide YY Y1 receptor gene in SK-N-MC cells

Peter J. Mannon, Lynn M. Kaiser

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

To identify effectors of neuropeptide Y Y1 receptor gene expression predicted by putative regulatory elements in its 5' flanking region, we examined the effects of several transcriptionally active agents on Y1 receptor mRNA levels and 125I-peptide YY binding capacity in SK-N-MC cells. Phorbol ester caused a rapid, transient 2.6-fold increase in Y1R mRNA levels. However, all trans-retinoic acid caused a rapid, sustained decrease in Y1 receptor mRNA levels (to 25% of control). Cycloheximide pretreatment did not attenuate the maximal inhibitory effect of retinoic acid, but it prolonged the time to achieve maximal efficacy. The retinoic acid effect was secondary to both a significant decrease in Y1 receptor mRNA stability and a decreased Y1 receptor gene transcription rate. Y1 receptor binding capacity was also significantly decreased after retinoic acid treatment (368 ± 25 vs. 496 ± 28 fmol/mg of protein for control). These data support a role for retinoic acid as an important agent regulating Y1 receptor gene expression and mediating Y1 receptor down-regulation.

Original languageEnglish (US)
Pages (from-to)20-25
Number of pages6
JournalJournal of Neurochemistry
Volume68
Issue number1
DOIs
StatePublished - Jan 1997
Externally publishedYes

Keywords

  • Neuropeptide Y
  • Peptide YY
  • Retinoic acid
  • SK-N-MC cells
  • Y1 receptor

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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