Retroviral-infection increases tumorigenic potential of MDA-MB-231 breast carcinoma cells by expanding an aldehyde dehydrogenase (ALDH1) positive stem-cell like population

Lauren J. Wegman-Points, Melissa L.T. Teoh-Fitzgerald, Gaowei Mao, Yueming Zhu, Melissa A. Fath, Douglas R. Spitz, Frederick E. Domann

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Retroviral transformation has been associated with pro-proliferative oncogenic signaling in human cells. The current study demonstrates that transduction of human breast carcinoma cells (MDA-MB231) with LXSN and QCXIP retroviral vectors causes significant increases in growth rate, clonogenic fraction, and aldehyde dehydrogenase-1 positive cells (ALDH1+), which is associated with increased steady-state levels of cancer stem cell populations. Furthermore, this retroviral-induced enhancement of cancer cell growth in vitro was also accompanied by a significant increase in xenograft tumor growth rate in vivo. The retroviral induced increases in cancer cell growth rate were partially inhibited by treatment with 100 U/ml polyethylene glycol-conjugated-(PEG)-superoxide dismutase and/or PEG-catalase. These results show that retroviral infection of MDA-MB231 human breast cancer cells is capable of enhancing cell proliferation and cancer stem cell populations as well as suggesting that modulation of reactive oxygen species-induced pro-survival signaling pathways may be involved inthese effects.

Original languageEnglish (US)
Pages (from-to)847-854
Number of pages8
JournalRedox Biology
Volume2
Issue number1
DOIs
StatePublished - 2014

Keywords

  • Aldehyde dehydrogenase positive
  • Antioxidant enzymes
  • Mammary cancer
  • Oxidative stress
  • Stem cells
  • Viral carcinogenesis

ASJC Scopus subject areas

  • Organic Chemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Retroviral-infection increases tumorigenic potential of MDA-MB-231 breast carcinoma cells by expanding an aldehyde dehydrogenase (ALDH1) positive stem-cell like population'. Together they form a unique fingerprint.

  • Cite this