Review of animal models designed to predict the potential allergenicity of novel proteins in genetically modified crops

G. S. Ladics, L. M.J. Knippels, A. H. Penninks, G. A. Bannon, R. E. Goodman, C. Herouet-Guicheney

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

The safety assessment of genetically modified crops involves the evaluation of the potential allergenicity of novel proteins by using several in silico and in vitro endpoints. In this publication, the variables and questions associated with the development of in vivo models are examined and several unpublished results are presented. Both rodent and non-rodent (dog and pig) models have been investigated using various routes of administration with purified proteins or food extracts, with or without the use of an adjuvant. The ideal model should be simple, reproducible across laboratories over time, specific and sensitive enough for distinguishing a threshold beyond which relevant allergenicity would be predicted and, for ranking proteins correlated with the allergic responses in humans, and acceptable under animal care. Preliminary data suggest that a few appear promising; however, further evaluation of these models is required. In particular, more extensive validation testing with additional allergenic and non-allergenic material should be performed before using them in the safety assessment of genetically modified crops.

Original languageEnglish (US)
Pages (from-to)212-224
Number of pages13
JournalRegulatory Toxicology and Pharmacology
Volume56
Issue number2
DOIs
StatePublished - Mar 1 2010

Keywords

  • Allergenicity
  • Animal model
  • Food allergy
  • Genetically modified food
  • IgE
  • Protein

ASJC Scopus subject areas

  • Toxicology

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