Revisiting protocols for the NMR analysis of bacterial metabolomes

Steven Halouska, Bo Zhang, Rosmarie Gaupp, Shulei Lei, Emily Snell, Robert J. Fenton, Raul G. Barletta, Greg A. Somerville, Robert Powers

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

Over the past decade, metabolomics has emerged as an important technique for systems biology. Measuring all the metabolites in a biological system provides an invaluable source of information to explore various cellular processes, and to investigate the impact of environmental factors and genetic modifications. Nuclear magnetic resonance (NMR) spectroscopy is an important method routinely employed in metabolomics. NMR provides comprehensive structural and quantitative information useful for metabolomics fingerprinting, chemometric analysis, metabolite identification and metabolic pathway construction. A successful metabolomics study relies on proper experimental protocols for the collection, handling, processing and analysis of metabolomics data. Critically, these protocols should eliminate or avoid biologicallyirrelevant changes to the metabolome. We provide a comprehensive description of our NMR-based metabolomics procedures optimized for the analysis of bacterial metabolomes. The technical details described within this manuscript should provide a useful guide to reliably apply our NMR-based metabolomics methodology to systems biology studies.

Original languageEnglish (US)
Pages (from-to)120-137
Number of pages18
JournalJournal of Integrated OMICS
Volume3
Issue number2
DOIs
StatePublished - Dec 2013

Keywords

  • Bacteria
  • Chemometrics
  • Metabolomics
  • Mycobacterium
  • NMR
  • Staphylococcus

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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