Rheological, in situ printability and cell viability analysis of hydrogels for muscle tissue regeneration

Srikanthan Ramesh, Sam Gerdes, Sharon Lau, Azadeh Mostafavi, Zhenyu Kong, Blake N. Johnson, Ali Tamayol, Prahalada Rao, Iris V. Rivero

Research output: Contribution to conferencePaper

Abstract

Advancements in additive manufacturing have made it possible to fabricate biologically relevant architectures from a wide variety of materials. Hydrogels have garnered increased attention for the fabrication of muscle tissue engineering constructs due to their resemblance to living tissue and ability to function as cell carriers. However, there is a lack of systematic approaches to screen bioinks based on their inherent properties, such as rheology, printability and cell viability. Furthermore, this study takes the critical first-step for connecting in-process sensor data with construct quality by studying the influence of printing parameters. Alginate-chitosan hydrogels were synthesized and subjected to a systematic rheological analysis. In situ print layer photography was utilized to identify the optimum printing parameters and also characterize the fabricated three-dimensional structures. Additionally, the scaffolds were seeded with C2C12 mouse myoblasts to test the suitability of the scaffolds for muscle tissue engineering. The results from the rheological analysis and print layer photography led to the development of a set of optimum processing conditions that produced a quality deposit while the cell viability tests indicated the suitability of the hydrogel for muscle tissue engineering applications.

Original languageEnglish (US)
Pages835-846
Number of pages12
StatePublished - 2020
Event29th Annual International Solid Freeform Fabrication Symposium - An Additive Manufacturing Conference, SFF 2018 - Austin, United States
Duration: Aug 13 2018Aug 15 2018

Conference

Conference29th Annual International Solid Freeform Fabrication Symposium - An Additive Manufacturing Conference, SFF 2018
CountryUnited States
CityAustin
Period8/13/188/15/18

Keywords

  • Bioprinting
  • C2C12 myoblasts
  • In-situ monitoring
  • Muscle regeneration
  • Printability

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Surfaces and Interfaces

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    Ramesh, S., Gerdes, S., Lau, S., Mostafavi, A., Kong, Z., Johnson, B. N., Tamayol, A., Rao, P., & Rivero, I. V. (2020). Rheological, in situ printability and cell viability analysis of hydrogels for muscle tissue regeneration. 835-846. Paper presented at 29th Annual International Solid Freeform Fabrication Symposium - An Additive Manufacturing Conference, SFF 2018, Austin, United States.