Rhizopus stolonifer sporangiospores were harvested with and without contact with water. The wet-harvested spores differed from the dry-harvested spores in that they: (i) incorporated 14CO2 into both tricarboxylic acid-soluble and -insoluble material; (ii) possessed polyribosomes; (iii) did not maintain viability during storage at room temperature; and (iv) gradually exhibited altered nutritional requirements for germination. Consequently, wet-harvested spores cannot be considered to be either native or dormant. Even brief (30 min) exposures to water during harvesting caused the spores to commence metabolic activity.
- Rhizopus stolonifer
- sporangiospores harvesting
- spores wet vs dry harvesting
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology