@article{c558f7b84194499da11d98fb6c294ef0,
title = "Rhodopseudomonas palustris CGA009 polyhydroxybutyrate production from a lignin aromatic and quantification via flow cytometry",
abstract = "Polyhydroxyalkanoates are biopolymers with the potential to replace petroleum-based plastics but are limited by higher productioncosts. For the first time, (i) polyhydroxybutyrate (PHB) was produced by the metabolically versatile Rhodopseudomonas palustris CGA009 from the lignin breakdown product p-coumarate as a renewable carbon source, and (ii) quantification of PHB via flow cytometry was conducted with this highly pigmented bacterium. PHB titer ranged from 0.08 to 0.41 g/L and yielded a 68.4% carbon conversion efficiency. An optimized protocol using either Nile Red or BODIPY 493/503 lipophilic stains yielded high linear fitness for fluorescence intensity with both PHB titer (R2 = 0.9384 and R2 = 0.9747 respectively) and cell count (R2 = 0.9383 and R2 = 0.9955 respectively). This quick and precise method for high-throughput quantification of both PHB and cell count coupled with the highest PHB titer from a lignin breakdown product to date from R. palustris make this study novel.",
keywords = "BODIPY, Flow cytometry, Lignin, Nile Red, Polyhydroxyalkanoates",
author = "Brandi Brown and Cheryl Immethun and Mark Wilkins and Rajib Saha",
note = "Funding Information: We would like to thank the Nebraska Center for Energy Sciences Research, University of Nebraska-Lincoln for funding this project [grant numbers 26-1217-0020-403 and 26-1217-0020-413]. Additionally, we extend thanks to Dr. You Zhou at the University of Nebraska-Lincoln Microscopy Core Facility and Mr. Dirk Anderson at the University of Nebraska-Lincoln Flow Cytometry Core Facility for providing guidance for running the experiments. We are also thankful to Anjeza Erickson at the Industrial Agricultural Products Center, University of Nebraska-Lincoln for providing assistance with GC?MS analysis. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. Funding Information: We would like to thank the Nebraska Center for Energy Sciences Research, University of Nebraska-Lincoln for funding this project [grant numbers 26-1217-0020-403 and 26-1217-0020-413 ]. Additionally, we extend thanks to Dr. You Zhou at the University of Nebraska-Lincoln Microscopy Core Facility and Mr. Dirk Anderson at the University of Nebraska-Lincoln Flow Cytometry Core Facility for providing guidance for running the experiments. We are also thankful to Anjeza Erickson at the Industrial Agricultural Products Center, University of Nebraska-Lincoln for providing assistance with GC–MS analysis. Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",
year = "2020",
month = sep,
doi = "10.1016/j.biteb.2020.100474",
language = "English (US)",
volume = "11",
journal = "Bioresource Technology Reports",
issn = "2589-014X",
publisher = "Elsevier Ltd",
}