RNA expression patterns in serum microvesicles from patients with glioblastoma multiforme and controls

Mikkel Noerholm, Leonora Balaj, Tobias Limperg, Afshin Salehi, Lin D. Zhu, Fred H. Hochberg, Xandra O. Breakefield, Bob S. Carter, Johan Skog

Research output: Contribution to journalArticlepeer-review

148 Scopus citations

Abstract

Background: RNA from exosomes and other microvesicles contain transcripts of tumour origin. In this study we sought to identify biomarkers of glioblastoma multiforme in microvesicle RNA from serum of affected patients.Methods: Microvesicle RNA from serum from patients with de-novo primary glioblastoma multiforme (N = 9) and normal controls (N = 7) were analyzed by microarray analysis. Samples were collected according to protocols approved by the Institutional Review Board. Differential expressions were validated by qRT-PCR in a separate set of samples (N = 10 in both groups).Results: Expression profiles of microvesicle RNA correctly separated individuals in two groups by unsupervised clustering. The most significant differences pertained to down-regulated genes (121 genes > 2-fold down) in the glioblastoma multiforme patient microvesicle RNA, validated by qRT-PCR on several genes. Overall, yields of microvesicle RNA from patients was higher than from normal controls, but the additional RNA was primarily of size < 500 nt. Gene ontology of the down-regulated genes indicated these are coding for ribosomal proteins and genes related to ribosome production.Conclusions: Serum microvesicle RNA from patients with glioblastoma multiforme has significantly down-regulated levels of RNAs coding for ribosome production, compared to normal healthy controls, but a large overabundance of RNA of unknown origin with size < 500 nt.

Original languageEnglish (US)
Article number22
JournalBMC cancer
Volume12
DOIs
StatePublished - Jan 17 2012
Externally publishedYes

Keywords

  • Biomarkers
  • Exosomes
  • Glioma
  • Microarray
  • Microvesicles
  • Serum

ASJC Scopus subject areas

  • Oncology
  • Genetics
  • Cancer Research

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