Role for a novel Usher protein complex in hair cell synaptic maturation

Marisa Zallocchi, Daniel T. Meehan, Duane Delimont, Joseph Rutledge, Michael Anne Gratton, John Flannery, Dominic Cosgrove

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The molecular mechanisms underlying hair cell synaptic maturation are not well understood. Cadherin-23 (CDH23), protocadherin-15 (PCDH15) and the very large G-protein coupled receptor 1 (VLGR1) have been implicated in the development of cochlear hair cell stereocilia, while clarin-1 has been suggested to also play a role in synaptogenesis. Mutations in CDH23, PCDH15, VLGR1 and clarin-1 cause Usher syndrome, characterized by congenital deafness, vestibular dysfunction and retinitis pigmentosa. Here we show developmental expression of these Usher proteins in afferent spiral ganglion neurons and hair cell synapses. We identify a novel synaptic Usher complex comprised of clarin-1 and specific isoforms of CDH23, PCDH15 and VLGR1. To establish the in vivo relevance of this complex, we performed morphological and quantitative analysis of the neuronal fibers and their synapses in the Clrn1-/- mouse, which was generated by incomplete deletion of the gene. These mice showed a delay in neuronal/synaptic maturation by both immunostaining and electron microscopy. Analysis of the ribbon synapses in Ames waltzer av3J mice also suggests a delay in hair cell synaptogenesis. Collectively, these results show that, in addition to the well documented role for Usher proteins in stereocilia development, Usher protein complexes comprised of specific protein isoforms likely function in synaptic maturation as well.

Original languageEnglish (US)
Article numbere30573
JournalPloS one
Volume7
Issue number2
DOIs
StatePublished - Feb 17 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

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    Zallocchi, M., Meehan, D. T., Delimont, D., Rutledge, J., Gratton, M. A., Flannery, J., & Cosgrove, D. (2012). Role for a novel Usher protein complex in hair cell synaptic maturation. PloS one, 7(2), [e30573]. https://doi.org/10.1371/journal.pone.0030573