Role of nitric oxide in disruption of the blood-brain barrier during acute hypertension

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50 Scopus citations

Abstract

The goal of this study was to determine the role of nitric oxide in disruption of the blood-brain barrier during acute hypertension. We examined the microcirculation of the cerebrum in vivo. Permeability of the blood-brain barrier was quantitated by the formation of venular leaky sites and clearance of fluorescent-labeled albumin (FITC-albumin) before and during phenylephrine-induced acute hypertension. We compared disruption of the blood-brain barrier during acute hypertension in untreated rats and in rats treated for 1 h with topical application ofNG-monomethyl-l-arginine (l-NMMA; 100 μM) orNG-nitro-l-arginine methyl ester (l-NAME; 100 μM). Under control conditions, no venular leaky sites were visible and clearance of FITC-albumin was minimal in untreated rats and in rats treated with topical application of nitric oxide synthase inhibitors. Phenylephrine (20 μg/kg/min for 5 min) infusion increased systemic arterial pressure by a similar magnitude in all groups of rats and produced disruption of the blood-brain barrier in venules. However, the magnitude of disruption of the blood-brain barrier during acute hypertension was significantly less in rats treated withl-NMMA (52% reduction in the clearance of FITC-albumin) andl-NAME (47% reduction in clearance of FITC-albumin). The findings of the present study suggest that synthesis/release of nitric oxide contributes to disruption of the blood-brain barrier during acute hypertension.

Original languageEnglish (US)
Pages (from-to)99-103
Number of pages5
JournalBrain Research
Volume686
Issue number1
DOIs
StatePublished - Jul 17 1995

Keywords

  • Fluorescein isothiocyanate albumin
  • Microcirculation
  • Pial venule
  • Rat
  • l-NAME
  • l-NMMA

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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