Promoters are regulatory elements that provide precise control of magnitude, specificity and duration of shRNA expression and gene silencing. The selection of a suitable promoter is therefore critical in the design of a shRNA expression system. We discuss the use of different promoters for shRNA expression, including constitutive promoters, inducible promoters, and tissue-/tumor-specific promoters. Recently, miRNA-based shRNAs have received increasing attention as a promising RNAi approach. We have therefore also summarized the use of a miRNA backbone to enhance gene silencing and to reduce toxicities. The application of miRNA-shRNA for combinatorial gene therapy is also discussed.
- Cell-specific promoters
- Combinatorial RNAi
- Constitutive promoters
- Inducible promoters
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)