Role of Promoters and Micro RNA Backbone for Efficient Gene Silencing

Feng Li, Ram I. Mahato

Research output: Chapter in Book/Report/Conference proceedingChapter


Promoters are regulatory elements that provide precise control of magnitude, specificity and duration of shRNA expression and gene silencing. The selection of a suitable promoter is therefore critical in the design of a shRNA expression system. We discuss the use of different promoters for shRNA expression, including constitutive promoters, inducible promoters, and tissue-/tumor-specific promoters. Recently, miRNA-based shRNAs have received increasing attention as a promising RNAi approach. We have therefore also summarized the use of a miRNA backbone to enhance gene silencing and to reduce toxicities. The application of miRNA-shRNA for combinatorial gene therapy is also discussed.

Original languageEnglish (US)
Title of host publicationAdvanced Delivery and Therapeutic Applications of RNAi
PublisherJohn Wiley and Sons
Number of pages26
ISBN (Print)9781119976868
StatePublished - Apr 26 2013
Externally publishedYes


  • Cell-specific promoters
  • Combinatorial RNAi
  • Constitutive promoters
  • Inducible promoters
  • MiRNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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