Role of the CD39/CD73 purinergic pathway in modulating arterial thrombosis in mice

Roman Covarrubias, Elena Chepurko, Adam Reynolds, Zachary M. Huttinger, Ryan Huttinger, Katherine Stanfill, Debra G. Wheeler, Tatiana Novitskaya, Simon C. Robson, Karen M. Dwyer, Peter J. Cowan, Richard J. Gumina

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Objective-Circulating blood cells and endothelial cells express ectonucleoside triphosphate diphosphohydrolase-1 (CD39) and ecto-5'-nucleotidase (CD73). CD39 hydrolyzes extracellular ATP or ADP to AMP. CD73 hydrolyzes AMP to adenosine. The goal of this study was to examine the interplay between CD39 and CD73 cascade in arterial thrombosis. Approach and Results-To determine how CD73 activity influences in vivo thrombosis, the time to ferric chloride-induced arterial thrombosis was measured in CD73-null mice. In response to 5% FeCl3, but not to 10% FeCl3, there was a significant decrease in the time to thrombosis in CD73-null mice compared with wild-Type mice. In mice overexpressing CD39, ablation of CD73 did not inhibit the prolongation in the time to thrombosis conveyed by CD39 overexpression. However, the CD73 inhibitor α-β-methylene-ADP nullified the prolongation in the time to thrombosis in human CD39 transgenic (hC39-Tg)/CD73-null mice. To determine whether hematopoietic-derived cells or endothelial cell CD39 activity regulates in vivo arterial thrombus, bone marrow transplant studies were conducted. FeCl3-induced arterial thrombosis in chimeric mice revealed a significant prolongation in the time to thrombosis in hCD39-Tg reconstituted wild-Type mice, but not on wild-Type reconstituted hCD39-Tg mice. Monocyte depletion with clodronate-loaded liposomes normalized the time to thrombosis in hCD39-Tg mice compared with hCD39-Tg mice treated with control liposomes, demonstrating that increased CD39 expression on monocytes protects against thrombosis. Conclusions-These data demonstrate that ablation of CD73 minimally effects in vivo thrombosis, but increased CD39 expression on hematopoietic-derived cells, especially monocytes, attenuates in vivo arterial thrombosis.

Original languageEnglish (US)
Pages (from-to)1809-1820
Number of pages12
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Issue number9
StatePublished - Sep 1 2016


  • Adenosine diphosphate
  • Monocytes
  • Nucleotidase
  • Platelet activation
  • Thrombosis

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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