Saccharomyces cerevisiae Ats1p interacts with Nap1p, a cytoplasmic protein that controls bud morphogenesis

Christina M. Shields, Rachel Taylor, Tara Nazarenus, Joseph Cheatle, Ann Hou, Audrey Tapprich, Alexis Haifley, Audrey L. Atkin

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Saccharomyces cerevisiae ATS1 (α-tubulin suppressor 1) was originally identified as a high-copy suppressor of class two α-tubulin mutations and was proposed to have a regulatory role in coordinating the microtubule state with the cell cycle. Here, we show that Ats1p interacts with Nap1p, a cytoplasmic protein that regulates the activity of the Cdc28p/C1b2p complex. Loss of Nap1p results in a delayed switch from polar to isotropic bud growth. The delayed switch results in elongated buds. Nap1p and Ats1p interact in two-hybrid and co-immunoprecipitation assays. Both nap1Δ and ats1Δ cells have a C1b2p-dependent elongated bud morphology. Deletion of ATS1 partially suppresses the elongated bud morphology and benomyl resistance of nap1Δ mutants. Our results suggest Atslp might regulate coordination of the microtubule state with the cell cycle through an interaction with Nap1p.

Original languageEnglish (US)
Pages (from-to)184-194
Number of pages11
JournalCurrent Genetics
Volume44
Issue number4
DOIs
StatePublished - Dec 2003

Keywords

  • Ats1p
  • Cell cycle
  • Clb2p
  • Morphogenesis checkpoint
  • Nap1p

ASJC Scopus subject areas

  • Genetics

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