@article{8e7576fb268a42e6b0a1b4ac6b38761a,
title = "Saccharomyces cerevisiae Ats1p interacts with Nap1p, a cytoplasmic protein that controls bud morphogenesis",
abstract = "Saccharomyces cerevisiae ATS1 (α-tubulin suppressor 1) was originally identified as a high-copy suppressor of class two α-tubulin mutations and was proposed to have a regulatory role in coordinating the microtubule state with the cell cycle. Here, we show that Ats1p interacts with Nap1p, a cytoplasmic protein that regulates the activity of the Cdc28p/C1b2p complex. Loss of Nap1p results in a delayed switch from polar to isotropic bud growth. The delayed switch results in elongated buds. Nap1p and Ats1p interact in two-hybrid and co-immunoprecipitation assays. Both nap1Δ and ats1Δ cells have a C1b2p-dependent elongated bud morphology. Deletion of ATS1 partially suppresses the elongated bud morphology and benomyl resistance of nap1Δ mutants. Our results suggest Atslp might regulate coordination of the microtubule state with the cell cycle through an interaction with Nap1p.",
keywords = "Ats1p, Cell cycle, Clb2p, Morphogenesis checkpoint, Nap1p",
author = "Shields, {Christina M.} and Rachel Taylor and Tara Nazarenus and Joseph Cheatle and Ann Hou and Audrey Tapprich and Alexis Haifley and Atkin, {Audrey L.}",
note = "Funding Information: Acknowledgements This material is based upon work supported by the National Science Foundation under grant 9874516 and a grant from the Layman Foundation. Any opinions, findings, conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation or the Layman Foundation. We would like to thank Drs. Phil James, Frank Solomon, David Kirkpatrick, Doug Kellogg and Raffael Schaffrath for providing plasmids, strains, antibodies and suggestions. We thank Dr. You Zhou and Michelle Mathiesen for technical assistance with microscopy and thank Drs. Kenneth Nickerson and Jacob Hornby for their helpful advice on the growth curve and dry cell weight experiments. We would also like to thank Drs. Steve Harris, Cathy Chia, Larry Harshman and John Osterman and members of the Atkin Laboratory for their critical reading and helpful suggestions during preparation of the manuscript.",
year = "2003",
month = dec,
doi = "10.1007/s00294-003-0442-z",
language = "English (US)",
volume = "44",
pages = "184--194",
journal = "Current Genetics",
issn = "0172-8083",
publisher = "Springer Verlag",
number = "4",
}