Sampling the skin transcriptome of the North Atlantic right whale

Jessalyn L. Ierardi; Annalaura Mancia; Jo Ellyn McMillan; Mats L. Lundqvist; Tracy A. Romano; John Pierce Wise; Gregory W. Warr; Robert W. Chapman

doi:10.1016/j.cbd.2009.01.004

Sampling the skin transcriptome of the North Atlantic right whale

Jessalyn L. Ierardi, Annalaura Mancia, Jo Ellyn McMillan, Mats L. Lundqvist, Tracy A. Romano, John Pierce Wise, Gregory W. Warr, Robert W. Chapman

Pharmacology & Experimental Neuroscience (PEN)

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

As an initial step in defining the transcriptome of the North Atlantic right whale (Eubalaena glacialis) and developing functional genomic tools to study right whale health at the molecular physiological level, a cDNA library has been constructed from a skin biopsy. 2496 randomly selected clones (expressed sequence tags, ESTs) have been sequenced, and genes identified as important in the response to stress and immune challenges have been cloned by targeted RT-PCR from skin cDNA. The analysis of the EST collection (archived at www.marinegenomics.org and GenBank) showed a 34.79% redundancy, yielding 1578 unigenes and 27 potential microsatellite markers. 96 genes were cloned by targeted PCR; moreover, 52 of these genes are stress and immune function related. A Gene Ontology analysis of the unigene collection indicates that the skin is a rich source of expressed genes with diverse functions, suggesting an important role in multiple physiological processes including those related to immunity and stress response.

Original language	English (US)
Pages (from-to)	154-158
Number of pages	5
Journal	Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume	4
Issue number	3
DOIs	https://doi.org/10.1016/j.cbd.2009.01.004
State	Published - Sep 2009

Keywords

Eubalaena glacialis
Expressed Sequence Tags (ESTs)
Immune response
Microsatellite
North Atlantic right whale
Skin

ASJC Scopus subject areas

Biochemistry
Physiology
Aquatic Science
Animal Science and Zoology
Molecular Biology
Genetics

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10.1016/j.cbd.2009.01.004

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title = "Sampling the skin transcriptome of the North Atlantic right whale",

abstract = "As an initial step in defining the transcriptome of the North Atlantic right whale (Eubalaena glacialis) and developing functional genomic tools to study right whale health at the molecular physiological level, a cDNA library has been constructed from a skin biopsy. 2496 randomly selected clones (expressed sequence tags, ESTs) have been sequenced, and genes identified as important in the response to stress and immune challenges have been cloned by targeted RT-PCR from skin cDNA. The analysis of the EST collection (archived at www.marinegenomics.org and GenBank) showed a 34.79% redundancy, yielding 1578 unigenes and 27 potential microsatellite markers. 96 genes were cloned by targeted PCR; moreover, 52 of these genes are stress and immune function related. A Gene Ontology analysis of the unigene collection indicates that the skin is a rich source of expressed genes with diverse functions, suggesting an important role in multiple physiological processes including those related to immunity and stress response.",

keywords = "Eubalaena glacialis, Expressed Sequence Tags (ESTs), Immune response, Microsatellite, North Atlantic right whale, Skin",

author = "Ierardi, {Jessalyn L.} and Annalaura Mancia and McMillan, {Jo Ellyn} and Lundqvist, {Mats L.} and Romano, {Tracy A.} and Wise, {John Pierce} and Warr, {Gregory W.} and Chapman, {Robert W.}",

note = "Funding Information: This research was supported by awards from the National Institute of Standards and Technology and by fellowship support from the College of Charleston to J.I. We gratefully acknowledge the help of Dr. Teri Rowles in providing the skin biopsy sample. This work constitutes scientific contribution no. 179 from the Sea Research Foundation, Inc. This study was performed under permit 998-1678-01 from the National Marine Fisheries Service, National Oceanic and Atmospheric Administration. ",

year = "2009",

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doi = "10.1016/j.cbd.2009.01.004",

language = "English (US)",

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AU - Mancia, Annalaura

AU - McMillan, Jo Ellyn

AU - Lundqvist, Mats L.

AU - Romano, Tracy A.

AU - Wise, John Pierce

AU - Warr, Gregory W.

AU - Chapman, Robert W.

N1 - Funding Information: This research was supported by awards from the National Institute of Standards and Technology and by fellowship support from the College of Charleston to J.I. We gratefully acknowledge the help of Dr. Teri Rowles in providing the skin biopsy sample. This work constitutes scientific contribution no. 179 from the Sea Research Foundation, Inc. This study was performed under permit 998-1678-01 from the National Marine Fisheries Service, National Oceanic and Atmospheric Administration.

PY - 2009/9

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N2 - As an initial step in defining the transcriptome of the North Atlantic right whale (Eubalaena glacialis) and developing functional genomic tools to study right whale health at the molecular physiological level, a cDNA library has been constructed from a skin biopsy. 2496 randomly selected clones (expressed sequence tags, ESTs) have been sequenced, and genes identified as important in the response to stress and immune challenges have been cloned by targeted RT-PCR from skin cDNA. The analysis of the EST collection (archived at www.marinegenomics.org and GenBank) showed a 34.79% redundancy, yielding 1578 unigenes and 27 potential microsatellite markers. 96 genes were cloned by targeted PCR; moreover, 52 of these genes are stress and immune function related. A Gene Ontology analysis of the unigene collection indicates that the skin is a rich source of expressed genes with diverse functions, suggesting an important role in multiple physiological processes including those related to immunity and stress response.

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Sampling the skin transcriptome of the North Atlantic right whale

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Keywords

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