Sanggenon C decreases tumor cell viability associated with proteasome inhibition

Hongbiao Huang, Ningning Liu, Kai Zhao, Chenchen Zhu, Xiaoyu Lu, Shujue Li, Wen Lian, Ping Zhou, Xiaoxian Dong, Canguo Zhao, Haiping Guo, Change Zhang, Changshan Yang, Guanmei Wen, Li Lu, Xiaofen Li, Lixia Guan, Chunjiao Liu, Xuejun Wang, Qing Ping DouJinbao Liu

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Several flavonoids have been reported to be proteasome inhibitors, but whether prenylated flavonoids are able to inhibit proteasome function remains unknown. We report for the first time that Sanggenon C, a natural prenylated flavonoid, inhibits tumor cellular proteasomal activity and cell viability. We found that (1) Sanggenon C inhibited tumor cell viability and induced cell cycle arrest at G0/G1 phase; (2) Sanggenon C inhibited the chymotrypsin-like activity of purified human 20S proteasome and 26S proteasome in H22 cell lysate, and Sanggenon C was able to dose-dependently accumulate ubiquitinated proteins and proteasome substrate protein p27; (3) Sanggenon C-induced proteasome inhibition occurred prior to cell death in murine H22 and P388 cell lines; (4) Sanggenon C induced death of human K562 cancer cells and primary cells isolated from leukemic patients. We conclude that Sanggenon C inhibits tumor cell viability via induction of cell cycle arrest and cell death, which is associated with its ability to inhibit the proteasome function and that proteasome inhibition by Sanggenon C at least partially contributes to the observed tumor cell growth-inhibitory activity.

Original languageEnglish (US)
Pages (from-to)1315-1325
Number of pages11
JournalFrontiers in Bioscience - Elite
Volume3 E
Issue number4
StatePublished - Jan 6 2011

Keywords

  • Cell cycle
  • Cell death
  • Flavonoid
  • Proteasome inhibitor
  • Sanggenon C

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Sanggenon C decreases tumor cell viability associated with proteasome inhibition'. Together they form a unique fingerprint.

  • Cite this

    Huang, H., Liu, N., Zhao, K., Zhu, C., Lu, X., Li, S., Lian, W., Zhou, P., Dong, X., Zhao, C., Guo, H., Zhang, C., Yang, C., Wen, G., Lu, L., Li, X., Guan, L., Liu, C., Wang, X., ... Liu, J. (2011). Sanggenon C decreases tumor cell viability associated with proteasome inhibition. Frontiers in Bioscience - Elite, 3 E(4), 1315-1325.