Scanning electrochemical and fluorescence microscopy for detection of reactive oxygen species in living cells

Sean E. Salamifar, Rebecca Y. Lai

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Understanding the complex nature of reactive oxygen species (ROS) requires the development of new analytical techniques that are rapid, sensitive, and capable of real-time detection of both intra- and extracellular ROS. In this regards, electrochemical techniques have shown to be promising when compared to conventional techniques used for ROS studies. The broad applicability of electrochemical techniques in quantifying ROS in living cells has resulted in the development of a wide range of electrochemical ROS sensors to date. However, relatively few of them have found applications in real biological studies; most have remained at the proof-of-concept stage. Hybrid optoelectrochemical approaches such as scanning electrochemical microscopy and fluorescence microscopy have shown to augment the detection capabilities of electrochemical techniques, rendering them suitable for studying complex biological systems.

Original languageEnglish (US)
Title of host publicationOxidative Stress
Subtitle of host publicationDiagnostics, Prevention, and Therapy Volume 2
EditorsSilvana Andreescu, Maria Hepel
PublisherAmerican Chemical Society
Pages415-430
Number of pages16
ISBN (Electronic)9780841231009
DOIs
StatePublished - 2015

Publication series

NameACS Symposium Series
Volume1200
ISSN (Print)0097-6156
ISSN (Electronic)1947-5918

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

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  • Cite this

    Salamifar, S. E., & Lai, R. Y. (2015). Scanning electrochemical and fluorescence microscopy for detection of reactive oxygen species in living cells. In S. Andreescu, & M. Hepel (Eds.), Oxidative Stress: Diagnostics, Prevention, and Therapy Volume 2 (pp. 415-430). (ACS Symposium Series; Vol. 1200). American Chemical Society. https://doi.org/10.1021/bk-2015-1200.ch017