SCID mice inoculated intracerebrally (1C) with preinfected (HIV) human macrophages develop brain pathology similar to that in humans with HIV encephalitis. This includes immimocytochemical detection in brain sections of HIV-infected macrophages and multinucleated giant cells, and significant astrogliosis compared to controls (mice injected 1C with uninfected human macrophages). These xenografts survive about 1 month. To develop a model of chronic HIV encephalitis and determine if chronically infected mice develop behavioral abnormalities similar to the human condition, we reinoculated SCID mice 1C every 4 weeks with either preinfected human macrophages (infected mice), uninfected human macrophages, or no inoculation; these groups were monitored for behavioral abnormalities. For 3 months after the first inoculation (total of 4 inoculations) each mouse was monitored on Stoelting activity monitors for 24 hours twice a month. There was no significant difference between groups in the amount of general activity during their normal day to day routine. However, the acquisition of a Morris maze problem by infected mice 3 1/2 months after the first inoculation was deficient in comparison to mice inoculated with uninfected macrophages or uninoculated mice. Ongoing studies are investigating the potential relationships between:(l) the histopathology such as the presence and amount of macrophages, HIV, and astrogliosis, (2) cytokine production (in situ detection and quantitative PCR for mllNA) such as TNF-alpha and others, and (3) the behavioral abnormalities.
|Original language||English (US)|
|State||Published - 1996|
ASJC Scopus subject areas
- Molecular Biology