Secondary enterovirus infection in the murine model of myocarditis. Pathologic and immunologic aspects

M. A. Beck, N. M. Chapman, B. M. McManus, J. C. Mullican, S. Tracy

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Abstract

Enteroviruses are implicated as etiologic agents in the inflammatory diseases myocarditis and polymyositis. In this report, we show that a previous enterovirus exposure in mice can influence development of myocardial inflammation with a second enteroviral exposure. Inoculation of 25-day-old male C3H/HeJ mice with 103 or 105 plaque-forming units (PFU) of infectious or ultra violet (UV)-inactivated coxsackievirus B2 (CVB2), followed by inoculation 28 days later with 105 PFU of a myocarditic variant of coxsackievirus B3 (CVB3-m) results in more intense myocardial inflammation and injury than is seen in age-matched mice inoculated with CVB3-m alone. More severe disease occurs with the lower primary dose of CVB2. Neutralizing antibody to CVB2 is detected early after primary inoculation and neutralizing antibody to CVB3 is first detected 5 days after secondary inoculation. In vitro proliferation of splenocytes from mice inoculated with one or both viruses occurs in response to both CVB2 and CVB3 antigens. We recently demonstrated that murine T cells are capable of recognizing an enterovirus group antigen. Thus cell-mediated immune responses to a conserved antigenic epitope(s) among the enteroviruses may be involved in the exacerbation of myocardial inflammatory disease during a second enterovirus infection. The secondary infection model described here may more accurately mirror virus-induced myocarditis in the human population because the majority of adults have been exposed to several enteroviruses before induction of disease.

Original languageEnglish (US)
Pages (from-to)669-681
Number of pages13
JournalAmerican Journal of Pathology
Volume136
Issue number3
StatePublished - 1990

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    Beck, M. A., Chapman, N. M., McManus, B. M., Mullican, J. C., & Tracy, S. (1990). Secondary enterovirus infection in the murine model of myocarditis. Pathologic and immunologic aspects. American Journal of Pathology, 136(3), 669-681.