TY - JOUR
T1 - Secretion of monocyte chemoattractant protein-1 by endothelial cells of the bovine corpus luteum
T2 - Regulation by cytokines but not prostaglandin F2α
AU - Cavicchio, Victoria A.
AU - Pru, James K.
AU - Davis, Benjamin S.
AU - Davis, John S.
AU - Rueda, B. R.
AU - Townson, David H.
PY - 2002/9
Y1 - 2002/9
N2 - Information regarding the regulation of monocyte chemoattractant protein-1 (MCP-1) in regression of the corpus luteum (CL) is limited. This study tested the hypothesis that endothelial cells derived from bovine CL are a source of MCP-1, and that proinflammatory cytokines, prostaglandin F2α (PGF2α), and progesterone regulate MCP-1 expression. Endothelial cells were treated without (Control) or with PGF2α (1 μM), TNFα (100 ng/ml), interferon-γ (IFNγ, 200 IU/ml), and TNFα + IFNγ for 24 and 48 h in the absence or presence of progesterone (P4, 250 ng/ml). Increases in MCP-1 mRNA and protein were observed in response to TNFα within 24 and 48 h of culture, respectively (P < 0.05). Interferon-γ stimulated (P < 0.05) both MCP-1 mRNA and protein after 24 h of culture, and this effect was also sustained through 48 h of culture (P < 0.05). Cotreatment of cultures with TNFα + IFNγ lead to further increases (P < 0.05) in MCP-1 in both 24- and 48-h cultures. Surprisingly, neither PGF2α nor P4 affected MCP-1 production. Subsequent experiments revealed that the endothelial cells lacked prostaglandin F2α receptor mRNA, and the MAPK pathway, although present and responsive to growth factor stimulation, was unresponsive to PGF2α stimulation. In summary, endothelial cells derived from bovine CL respond to TNFα and IFNγ stimulation with an increase in MCP-1 secretion. In contrast, neither PGF2α nor P4 directly influenced endothelial expression of MCP-1. These results suggest that cytokines stimulate the synthesis of MCP-1 observed during PGF2α-induced luteal regression.
AB - Information regarding the regulation of monocyte chemoattractant protein-1 (MCP-1) in regression of the corpus luteum (CL) is limited. This study tested the hypothesis that endothelial cells derived from bovine CL are a source of MCP-1, and that proinflammatory cytokines, prostaglandin F2α (PGF2α), and progesterone regulate MCP-1 expression. Endothelial cells were treated without (Control) or with PGF2α (1 μM), TNFα (100 ng/ml), interferon-γ (IFNγ, 200 IU/ml), and TNFα + IFNγ for 24 and 48 h in the absence or presence of progesterone (P4, 250 ng/ml). Increases in MCP-1 mRNA and protein were observed in response to TNFα within 24 and 48 h of culture, respectively (P < 0.05). Interferon-γ stimulated (P < 0.05) both MCP-1 mRNA and protein after 24 h of culture, and this effect was also sustained through 48 h of culture (P < 0.05). Cotreatment of cultures with TNFα + IFNγ lead to further increases (P < 0.05) in MCP-1 in both 24- and 48-h cultures. Surprisingly, neither PGF2α nor P4 affected MCP-1 production. Subsequent experiments revealed that the endothelial cells lacked prostaglandin F2α receptor mRNA, and the MAPK pathway, although present and responsive to growth factor stimulation, was unresponsive to PGF2α stimulation. In summary, endothelial cells derived from bovine CL respond to TNFα and IFNγ stimulation with an increase in MCP-1 secretion. In contrast, neither PGF2α nor P4 directly influenced endothelial expression of MCP-1. These results suggest that cytokines stimulate the synthesis of MCP-1 observed during PGF2α-induced luteal regression.
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U2 - 10.1210/en.2002-220388
DO - 10.1210/en.2002-220388
M3 - Article
C2 - 12193574
AN - SCOPUS:0036721074
SN - 0013-7227
VL - 143
SP - 3582
EP - 3589
JO - Endocrinology
JF - Endocrinology
IS - 9
ER -