TY - JOUR
T1 - Secretion of recombinant human fibrinogen by the murine mammary gland
AU - Butler, Stephen
AU - O'Sickey, Tanya
AU - Lord, Susan
AU - Lubon, Henryk
AU - Gwazdauskas, Francis
AU - Velander, William
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2004/10
Y1 - 2004/10
N2 - Transgenic animals secreting individual chains and assembled fibrinogen were produced to evaluate the capacity of the mammary gland for maximizing assembly, glycosylation and secretion of recombinant human fibrinogen (rhfib). Transgenes were constructed from the 4.1kbp murine Whey Acidic Protein promoter (mWAP) and the three cDNAs coding for the Aα, Bβ and γ fibrinogen chains. Transgenic mice secreted fully assembled fibrinogen into milk at concentrations between 10 and 200 μg/ml, with total secretion of subunits approaching 700 μg/ml in milk. Partially purified fibrinogen was shown to form a visible and stable clot after treatment with human thrombin and factor XIII. The level of assembled fibrinogen was proportional to the lowest amount of subunit produced where both the Bβ and γ chains were rate limiting. Both the Bβ and γ chains were glycosylated when co-expressed and the degree of saccharide maturation was dependent on expression level, with processing preferred for γ chains over Bβ chains. Also, the subunit complexes γ 2, A αγ2 and the individual subunits Aα, Bβ and γ were found as secretion products. When the Bβ was secreted individually, the glycosylation profile of the molecule was of a mature complex saccharide indicating recognition of the molecule by the glycosylation pathway without association with other fibrinogen chains. To date secretion of Bβ chain has been not observed in any cell type, suggesting that the secretion pathway in mammary epithelia is less restrictive than that occurring in hepatocytes and other cells previously used to study fibrinogen assembly.
AB - Transgenic animals secreting individual chains and assembled fibrinogen were produced to evaluate the capacity of the mammary gland for maximizing assembly, glycosylation and secretion of recombinant human fibrinogen (rhfib). Transgenes were constructed from the 4.1kbp murine Whey Acidic Protein promoter (mWAP) and the three cDNAs coding for the Aα, Bβ and γ fibrinogen chains. Transgenic mice secreted fully assembled fibrinogen into milk at concentrations between 10 and 200 μg/ml, with total secretion of subunits approaching 700 μg/ml in milk. Partially purified fibrinogen was shown to form a visible and stable clot after treatment with human thrombin and factor XIII. The level of assembled fibrinogen was proportional to the lowest amount of subunit produced where both the Bβ and γ chains were rate limiting. Both the Bβ and γ chains were glycosylated when co-expressed and the degree of saccharide maturation was dependent on expression level, with processing preferred for γ chains over Bβ chains. Also, the subunit complexes γ 2, A αγ2 and the individual subunits Aα, Bβ and γ were found as secretion products. When the Bβ was secreted individually, the glycosylation profile of the molecule was of a mature complex saccharide indicating recognition of the molecule by the glycosylation pathway without association with other fibrinogen chains. To date secretion of Bβ chain has been not observed in any cell type, suggesting that the secretion pathway in mammary epithelia is less restrictive than that occurring in hepatocytes and other cells previously used to study fibrinogen assembly.
KW - Mammary gland expression
KW - Recombinant human fibrinogen
KW - Transgenic mouse
KW - WAP promoter
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U2 - 10.1007/s11248-004-9589-8
DO - 10.1007/s11248-004-9589-8
M3 - Article
C2 - 15587268
AN - SCOPUS:7244238182
VL - 13
SP - 437
EP - 450
JO - Transgenic Research
JF - Transgenic Research
SN - 0962-8819
IS - 5
ER -