Selective expression of the small GTPase RhoB in the early developing mouse lens

Rupalatha Maddala, You Wei Peng, P. Vasantha Rao

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

This report describes the expression and distribution pattern of RhoB GTPase in the developing mouse lens. RhoB expression was confirmed by sequencing an reverse transcriptase-polymerase chain reaction-generated DNA fragment of RhoB. Immunohistochemical analysis of RhoB revealed expression in the lens vesicle (both anterior and posterior vesicle) at embryonic day (E) 11.5, and in the epithelium and primary fibers of the E14.5 lens. Compared with the neonatal stage (day 1), where RhoB is detected in the entire lens (epithelium, primary, and secondary fibers), expression of this protein is restricted to the epithelial and outer cortical secondary fibers in postnatal lenses (from day 7 to day18). Interestingly, in E11.5 and E14.5 lenses, RhoB is localized predominantly in the lens, but not detectable in the retina, cornea, or other ocular tissues. RhoB expression appears to be down-regulated in the postnatal lens with concomitant up-regulation in the retina and cornea, compared with earlier stages of development (eyes of Ell.5, E14.5, and neonatal mice). This study reveals the selective expression of RhoB in the lens during early eye development and suggests a potential role for this small GTPase in cytoskeletal reorganization associated with lens epithelial cell elongation and differentiation.

Original languageEnglish (US)
Pages (from-to)534-537
Number of pages4
JournalDevelopmental Dynamics
Volume222
Issue number3
DOIs
Publication statusPublished - Nov 20 2001

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Keywords

  • Cytoskeleton
  • Development
  • Differentiation and growth
  • Elongation
  • Eye
  • Growth factors
  • Human
  • Lens
  • Mouse
  • Retina
  • RhoB GTPase

ASJC Scopus subject areas

  • Developmental Biology

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