Selective transmission of R5-tropic HIV type 1 from dendritic cells to resting CD4+ T cells

S. A. David, M. S. Smith, G. J. Lopez, I. Adany, S. Mukherjee, S. Buch, M. M. Goodenow, O. Narayan

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

In an in vitro coculture model of monocyte-derived, cultured human dendritic cells (DC) with autologous CD4+ resting T cells, CCR5 (R5)-tropic strains of HIV-1, but not CXCR4 (X4)-tropic strains, were transmitted to resting CD4+ T cells, leading to prolific viral output, although DC were susceptible to infection with either strain. Macrophages, which were also infectable with either R5- or X4-tropic strains, did not transmit infection to CD4+ cells. Highly productive HIV infection in this model appeared to be a consequence of heterokaryotic syncytium formation between infected DC and T cells since syncytia formation developed only in R5-infected DC/CD4+ cocultures. These results suggested that the unique microenvironment derived from the fusion between the infected DC and CD4+ cell was highly permissive and selective for replication of R5-tropic viruses. The apparent selectivity for R5-tropic strains in such syncytia was attributable neither to differential DC-mediated activation nor to selective modulation of induction of α- or β-chemokines in the infected DC. This model of HIV replication may provide useful insights into in vitro correlates of HIV pathogenicity.

Original languageEnglish (US)
Pages (from-to)59-68
Number of pages10
JournalAIDS Research and Human Retroviruses
Volume17
Issue number1
DOIs
StatePublished - Jan 1 2001

ASJC Scopus subject areas

  • Immunology
  • Virology
  • Infectious Diseases

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    David, S. A., Smith, M. S., Lopez, G. J., Adany, I., Mukherjee, S., Buch, S., Goodenow, M. M., & Narayan, O. (2001). Selective transmission of R5-tropic HIV type 1 from dendritic cells to resting CD4+ T cells. AIDS Research and Human Retroviruses, 17(1), 59-68. https://doi.org/10.1089/088922201750056799