Sepsis induced by cecal ligation and puncture

Haitao Wen

Research output: Chapter in Book/Report/Conference proceedingChapter

34 Scopus citations


Despite advances in intensive care unit interventions, including the use of specific antibiotics and anti-inflammation treatment, sepsis with concomitant multiple organ failure is the most common cause of death in many acute care units. In order to understand the mechanisms of clinical sepsis and develop effective therapeutic modalities, there is a need to use effective experimental models that faithfully replicate what occurs in patients with sepsis. Several models are commonly used to study sepsis, including intravenous endotoxin challenge, injection of live organisms into the peritoneal cavity, establishing abscesses in the extremities, and the induction of polymicrobial peritonitis via cecal ligation and puncture (CLP). Here, we describe the surgery procedure of CLP in mice, which has been proposed to closely replicate the nature and course of clinical sepsis in humans.

Original languageEnglish (US)
Title of host publicationMouse Models of Innate Immunity
Subtitle of host publicationMethods and Protocols
EditorsIrving C. Allen
Number of pages8
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • CLP
  • Cecal ligation and puncture
  • Cytokine
  • Macrophage
  • Sepsis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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