Sequence analysis of the RNA polymerase gene of foot-and-mouth disease virus serotype Asia 1

George Manju, Venkataramanan Ramamurthy, Pattnaik Bramhadev, Sanyal Aniket, C. B. Gurumurthy, Hemadri Divakar, Tosh Chakradhar

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The complete nucleotide (nt.) sequence of the RNA polymerase (3D) gene and 81 nt. in the 3′-untranslated region of foot-and-mouth disease virus (FMDV) serotype Asia1 (IND63/72) was determined and compared with the sequence of other FMDV serotypes. The 3D genomic region was 1410 nt. long encoding 470 amino acids with an inframe stop codon (TAA) at nt. position 1411-1413. The deduced amino acid sequence of the protein showed 8 conserved motifs as reported in other picornaviruses, 2 of which are 100% identical across the serotypes. Antigenic regions in the polymerase protein were predicted and found to be located at the N-terminus of the protein. The phylogenetic analysis showed that the FMD viruses were segregated into different clusters based on geographical origin; the Asia1 virus did not cluster tightly with any of the geographical groups.

Original languageEnglish (US)
Article number281660
Pages (from-to)21-26
Number of pages6
JournalVirus Genes
Volume22
Issue number1
DOIs
StatePublished - 2001

Keywords

  • 3D gene sequence
  • Evolutionary distance
  • FMDV
  • Serotype Asial

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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