Sequence analysis of the RNA polymerase gene of foot-and-mouth disease virus serotype Asia 1

George Manju; Venkataramanan Ramamurthy; Pattnaik Bramhadev; Sanyal Aniket; C. B. Gurumurthy; Hemadri Divakar; Tosh Chakradhar

doi:10.1023/A:1008174100886

Sequence analysis of the RNA polymerase gene of foot-and-mouth disease virus serotype Asia 1

George Manju, Venkataramanan Ramamurthy, Pattnaik Bramhadev, Sanyal Aniket, C. B. Gurumurthy, Hemadri Divakar, Tosh Chakradhar

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The complete nucleotide (nt.) sequence of the RNA polymerase (3D) gene and 81 nt. in the 3′-untranslated region of foot-and-mouth disease virus (FMDV) serotype Asia1 (IND63/72) was determined and compared with the sequence of other FMDV serotypes. The 3D genomic region was 1410 nt. long encoding 470 amino acids with an inframe stop codon (TAA) at nt. position 1411-1413. The deduced amino acid sequence of the protein showed 8 conserved motifs as reported in other picornaviruses, 2 of which are 100% identical across the serotypes. Antigenic regions in the polymerase protein were predicted and found to be located at the N-terminus of the protein. The phylogenetic analysis showed that the FMD viruses were segregated into different clusters based on geographical origin; the Asia1 virus did not cluster tightly with any of the geographical groups.

Original language	English (US)
Article number	281660
Pages (from-to)	21-26
Number of pages	6
Journal	Virus Genes
Volume	22
Issue number	1
DOIs	https://doi.org/10.1023/A:1008174100886
State	Published - 2001
Externally published	Yes

Keywords

3D gene sequence
Evolutionary distance
FMDV
Serotype Asial

ASJC Scopus subject areas

Molecular Biology
Genetics
Virology

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10.1023/A:1008174100886

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title = "Sequence analysis of the RNA polymerase gene of foot-and-mouth disease virus serotype Asia 1",

abstract = "The complete nucleotide (nt.) sequence of the RNA polymerase (3D) gene and 81 nt. in the 3′-untranslated region of foot-and-mouth disease virus (FMDV) serotype Asia1 (IND63/72) was determined and compared with the sequence of other FMDV serotypes. The 3D genomic region was 1410 nt. long encoding 470 amino acids with an inframe stop codon (TAA) at nt. position 1411-1413. The deduced amino acid sequence of the protein showed 8 conserved motifs as reported in other picornaviruses, 2 of which are 100% identical across the serotypes. Antigenic regions in the polymerase protein were predicted and found to be located at the N-terminus of the protein. The phylogenetic analysis showed that the FMD viruses were segregated into different clusters based on geographical origin; the Asia1 virus did not cluster tightly with any of the geographical groups.",

keywords = "3D gene sequence, Evolutionary distance, FMDV, Serotype Asial",

author = "George Manju and Venkataramanan Ramamurthy and Pattnaik Bramhadev and Sanyal Aniket and Gurumurthy, {C. B.} and Hemadri Divakar and Tosh Chakradhar",

year = "2001",

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AU - Manju, George

AU - Ramamurthy, Venkataramanan

AU - Bramhadev, Pattnaik

AU - Aniket, Sanyal

AU - Gurumurthy, C. B.

AU - Divakar, Hemadri

AU - Chakradhar, Tosh

PY - 2001

Y1 - 2001

N2 - The complete nucleotide (nt.) sequence of the RNA polymerase (3D) gene and 81 nt. in the 3′-untranslated region of foot-and-mouth disease virus (FMDV) serotype Asia1 (IND63/72) was determined and compared with the sequence of other FMDV serotypes. The 3D genomic region was 1410 nt. long encoding 470 amino acids with an inframe stop codon (TAA) at nt. position 1411-1413. The deduced amino acid sequence of the protein showed 8 conserved motifs as reported in other picornaviruses, 2 of which are 100% identical across the serotypes. Antigenic regions in the polymerase protein were predicted and found to be located at the N-terminus of the protein. The phylogenetic analysis showed that the FMD viruses were segregated into different clusters based on geographical origin; the Asia1 virus did not cluster tightly with any of the geographical groups.

AB - The complete nucleotide (nt.) sequence of the RNA polymerase (3D) gene and 81 nt. in the 3′-untranslated region of foot-and-mouth disease virus (FMDV) serotype Asia1 (IND63/72) was determined and compared with the sequence of other FMDV serotypes. The 3D genomic region was 1410 nt. long encoding 470 amino acids with an inframe stop codon (TAA) at nt. position 1411-1413. The deduced amino acid sequence of the protein showed 8 conserved motifs as reported in other picornaviruses, 2 of which are 100% identical across the serotypes. Antigenic regions in the polymerase protein were predicted and found to be located at the N-terminus of the protein. The phylogenetic analysis showed that the FMD viruses were segregated into different clusters based on geographical origin; the Asia1 virus did not cluster tightly with any of the geographical groups.

KW - 3D gene sequence

KW - Evolutionary distance

KW - FMDV

KW - Serotype Asial

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Sequence analysis of the RNA polymerase gene of foot-and-mouth disease virus serotype Asia 1

Abstract

Keywords

ASJC Scopus subject areas

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