Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64A

Lisa A. Fitzgerald, Michael V. Graves, Xiao Li, Tamara Feldblyum, William C. Nierman, James L. Van Etten

Research output: Contribution to journalArticle

63 Scopus citations

Abstract

Viruses NY-2A and AR158, members of the family Phycodnaviridae, genus Chlorovirus, infect the fresh water, unicellular, eukaryotic, chlorella-like green alga, Chlorella NC64A. The 368,683-bp genome of NY-2A and the 344,690-bp genome of AR158 are the two largest chlorella virus genomes sequenced to date; NY-2A contains 404 putative protein-encoding and 7 tRNA-encoding genes and AR158 contains 360 putative protein-encoding and 6 tRNA-encoding genes. The protein-encoding genes are almost evenly distributed on both strands, and intergenic space is minimal. Two of the NY-2A genes encode inteins, the large subunit of ribonucleotide reductase and a superfamily II helicase. These are the first inteins to be detected in the chlorella viruses. Approximately 40% of the viral gene products resemble entries in the public databases, including some that are unexpected for a virus. These include GDP-d-mannose dehydratase, fucose synthase, aspartate transcarbamylase, Ca++ transporting ATPase and ubiquitin. Comparison of NY-2A and AR158 protein-encoding genes with the prototype chlorella virus PBCV-1 indicates that 85% of the genes are present in all three viruses.

Original languageEnglish (US)
Pages (from-to)472-484
Number of pages13
JournalVirology
Volume358
Issue number2
DOIs
StatePublished - Feb 20 2007

Keywords

  • Chlorella viruses
  • Genome sequence
  • Phycodnaviridae
  • Virus AR158
  • Virus NY-2A

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64A'. Together they form a unique fingerprint.

  • Cite this