Sequence Dependence of Reversible CENP-A Nucleosome Translocation

Micah P. Stumme-Diers, Thomas Stormberg, Yuri L. Lyubchenko

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Division of sister chromatids during mitosis relies on the proper formation of a kinetochore at the centromere. This specialized locus is defined by the presence of a histone H3 variant called centromere protein A (CENP-A), which is critical for the maintenance and function of the centromere. Another characteristic of centromeres are DNA motifs, such as alpha-satellite in humans, which differ between species but seemingly play an important role in centromere function. We recently reported on the highly dynamic behavior of CENP-A nucleosomes as captured using high-speed time-lapse atomic force microscopy (HS-AFM). One of the dynamic properties unique to CENP-A containing nucleosomes is the long-range translocation of the histone core along the DNA substrate. The process takes places in a stepwise fashion with the core stalling at various points along the substrate, suggesting a sequence dependence of the process. We analyzed the GC content of this sequence and found a correlation between high GC content and stalling of the CENP-A histone core. These results are presented below.

Original languageEnglish (US)
Title of host publicationBiomotors and their Nanobiotechnology Applications
PublisherCRC Press
Pages173-178
Number of pages6
ISBN (Electronic)9780429511943
ISBN (Print)9780367196134
DOIs
StatePublished - Jan 1 2023

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Engineering

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