Serologic and molecular studies of two kindreds expressing recombinant HLA DR1/DR2 haplotypes

James Wisecarver, Shirley Shephed, Kirk Beisel, Ronald Rubocki

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

We have recently identified two unrelated kindreds in which DR1 and DR2 co‐segregate as a single haplotype spanning several generations. Serology, PCR‐RFLP and sequence‐specific oligonucleotide probes were used to characterize the presence and segregation of the DRB1 and DRB5 loci in these two kindreds. In both families, the recombination resulted in co‐expression of a DRB1 locus that encoded a DR1 serologic phenotype and a DRB5 locus that encoded a “short” DR2 serologic phenotype. One of these kindreds co‐expressed the DR1 and DR15 (DRB5*01). The other kindred co‐expressed the DR1 and DR16 (DRB5*02). Our data indicate that the recombination event occurred in the region between the DRB1 locus and the DRB5 locus. This recombinant haplotype produces a DR2 phenotype which lacks the epitopes normally encoded by the DRB1 locus, resulting in a serologic “short” antigen. The clinical significance of such a recombinational event becomes evident when patients with such a genotype require allogeneic bone marrow transplantation. 1993 Blackwell Munksgaard

Original languageEnglish (US)
Pages (from-to)148-154
Number of pages7
JournalTissue Antigens
Volume41
Issue number3
DOIs
StatePublished - Mar 1993

Keywords

  • DRB1
  • DRB5
  • HLA‐DR recombinant
  • HLA‐DR triplet
  • PCR‐RFLP
  • PCR‐SSOP

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

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