TY - JOUR
T1 - Serotonin is essential for eye regeneration in planaria Schmidtea mediterranea
AU - Sarkar, Arunabha
AU - Mukundan, Namita
AU - Sowndarya, Sai
AU - Dubey, Vinay Kumar
AU - Babu, Rosana
AU - Lakshmanan, Vairavan
AU - Rangiah, Kannan
AU - Panicker, Mitradas M.
AU - Palakodeti, Dasaradhi
AU - Subramanian, Sabarinath Peruvemba
AU - Subramanian, Ramaswamy
N1 - Funding Information:
We are thankful to Kiyokazu Agata, RIKEN, Japan, for providing anti‐arrestin antibody; Dr Swapna Nandakumar for proofing and language correction of manuscript; Souradeep Sarkar for proofing; Mass Spectrometry Facility (Metabolomics) and Center for Imaging and Flow Cytometry Facility at CCAMP, Bangalore; NCBS‐TIFR for fellowship awarded to AS, NM, and VKD; Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, for postdoctoral fellowship awarded to SPS; and Department of Science and Technology (DST), Ministry of Science and Technology, Government of India, for Swarnajayanti Fellowship awarded to DP. This work was supported by DST Swarnajayanti Fellowship (DST/SJF/LSA‐02/2015‐16) and core funds of Institute for Stem Cell Science and Regenerative Medicine.
Funding Information:
We are thankful to Kiyokazu Agata, RIKEN, Japan, for providing anti-arrestin antibody; Dr Swapna Nandakumar for proofing and language correction of manuscript; Souradeep Sarkar for proofing; Mass Spectrometry Facility (Metabolomics) and Center for Imaging and Flow Cytometry Facility at CCAMP, Bangalore; NCBS-TIFR for fellowship awarded to AS, NM, and VKD; Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India, for postdoctoral fellowship awarded to SPS; and Department of Science and Technology (DST), Ministry of Science and Technology, Government of India, for Swarnajayanti Fellowship awarded to DP. This work was supported by DST Swarnajayanti Fellowship (DST/SJF/LSA-02/2015-16) and core funds of Institute for Stem Cell Science and Regenerative Medicine.
Publisher Copyright:
© 2019 Federation of European Biochemical Societies
PY - 2019/11/1
Y1 - 2019/11/1
N2 - Planaria is an ideal system to study factors involved in regeneration and tissue homeostasis. Little is known about the role of metabolites and small molecules in stem cell maintenance and lineage specification in planarians. Using liquid chromatography and mass spectrometry (LC-MS)-based quantitative metabolomics, we determined the relative levels of metabolites in stem cells, progenitors, and differentiated cells of the planarian Schmidtea mediterranea. Tryptophan and its metabolic product serotonin are significantly enriched in stem cells and progenitor population. Serotonin biosynthesis in these cells is brought about by a noncanonical enzyme, phenylalanine hydroxylase. Knockdown of Smed-pah leads to complete disappearance of eyes in regenerating planaria, while exogenous supply of serotonin and its precursor rescues the eyeless phenotype. Our results demonstrate a key role for serotonin in eye regeneration.
AB - Planaria is an ideal system to study factors involved in regeneration and tissue homeostasis. Little is known about the role of metabolites and small molecules in stem cell maintenance and lineage specification in planarians. Using liquid chromatography and mass spectrometry (LC-MS)-based quantitative metabolomics, we determined the relative levels of metabolites in stem cells, progenitors, and differentiated cells of the planarian Schmidtea mediterranea. Tryptophan and its metabolic product serotonin are significantly enriched in stem cells and progenitor population. Serotonin biosynthesis in these cells is brought about by a noncanonical enzyme, phenylalanine hydroxylase. Knockdown of Smed-pah leads to complete disappearance of eyes in regenerating planaria, while exogenous supply of serotonin and its precursor rescues the eyeless phenotype. Our results demonstrate a key role for serotonin in eye regeneration.
KW - LC-MS
KW - eye regeneration
KW - phenylalanine hydroxylase
KW - planaria
KW - serotonin
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U2 - 10.1002/1873-3468.13607
DO - 10.1002/1873-3468.13607
M3 - Article
C2 - 31529697
AN - SCOPUS:85074406913
SN - 0014-5793
VL - 593
SP - 3198
EP - 3209
JO - FEBS Letters
JF - FEBS Letters
IS - 22
ER -