Serum-induced membrane translocation of Nm23H1 in 95D cells

Li Tan, Ping Li, Xin Hui Sun, Chun Yang Li, Chang Tong, Jin Fan, Yun Song Zhu

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose: To explore the cellular distribution of Nm23H1 in human giant-cell lung carcinoma 95D cells. Methods: We used Immunofluorescence and Western blotting to analysis the distribution of Nm23H1 in 95D cells treated with serum after serum starvation for 24 hours. Results: Serum stimulation ould induced lamellipodia formation in 95D cells. Most of Nm23H1 existed in cytosol, but there was significant Nm23H1 staining in new formed lamellipodia. Using subcellular fractionation and Western blotting, we confirmed that Nm23H1 could be translocated to membrane after serum stimulation, which happened rapidly, peaked at about 30 minutes, and slightly reduced to above basal level at 8 hours. Conclusions: Our results indicated that Nm23H1 transloction is associated with the formation of lamellipodia, which may provide a clue to further elucidate the role of Nm23H1 played in tumor suppression.

Original languageEnglish (US)
Pages (from-to)255-258
Number of pages4
JournalFudan University Journal of Medical Sciences
Volume32
Issue number3
StatePublished - May 2005

Keywords

  • Lamellipodia
  • Membrane translocation
  • Nm23H1

ASJC Scopus subject areas

  • Medicine(all)

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