Purpose: To explore the cellular distribution of Nm23H1 in human giant-cell lung carcinoma 95D cells. Methods: We used Immunofluorescence and Western blotting to analysis the distribution of Nm23H1 in 95D cells treated with serum after serum starvation for 24 hours. Results: Serum stimulation ould induced lamellipodia formation in 95D cells. Most of Nm23H1 existed in cytosol, but there was significant Nm23H1 staining in new formed lamellipodia. Using subcellular fractionation and Western blotting, we confirmed that Nm23H1 could be translocated to membrane after serum stimulation, which happened rapidly, peaked at about 30 minutes, and slightly reduced to above basal level at 8 hours. Conclusions: Our results indicated that Nm23H1 transloction is associated with the formation of lamellipodia, which may provide a clue to further elucidate the role of Nm23H1 played in tumor suppression.
|Original language||English (US)|
|Number of pages||4|
|Journal||Fudan University Journal of Medical Sciences|
|State||Published - May 2005|
- Membrane translocation
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