Serum-induced membrane translocation of Nm23H1 in 95D cells

Li Tan; Ping Li; Xin Hui Sun; Chun Yang Li; Chang Tong; Jin Fan; Yun Song Zhu

Serum-induced membrane translocation of Nm23H1 in 95D cells

Li Tan, Ping Li, Xin Hui Sun, Chun Yang Li, Chang Tong, Jin Fan, Yun Song Zhu

Research output: Contribution to journal › Article › peer-review

Abstract

Purpose: To explore the cellular distribution of Nm23H1 in human giant-cell lung carcinoma 95D cells. Methods: We used Immunofluorescence and Western blotting to analysis the distribution of Nm23H1 in 95D cells treated with serum after serum starvation for 24 hours. Results: Serum stimulation ould induced lamellipodia formation in 95D cells. Most of Nm23H1 existed in cytosol, but there was significant Nm23H1 staining in new formed lamellipodia. Using subcellular fractionation and Western blotting, we confirmed that Nm23H1 could be translocated to membrane after serum stimulation, which happened rapidly, peaked at about 30 minutes, and slightly reduced to above basal level at 8 hours. Conclusions: Our results indicated that Nm23H1 transloction is associated with the formation of lamellipodia, which may provide a clue to further elucidate the role of Nm23H1 played in tumor suppression.

Original language	English (US)
Pages (from-to)	255-258
Number of pages	4
Journal	Fudan University Journal of Medical Sciences
Volume	32
Issue number	3
State	Published - May 2005

Keywords

Lamellipodia
Membrane translocation
Nm23H1

ASJC Scopus subject areas

Medicine(all)

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title = "Serum-induced membrane translocation of Nm23H1 in 95D cells",

abstract = "Purpose: To explore the cellular distribution of Nm23H1 in human giant-cell lung carcinoma 95D cells. Methods: We used Immunofluorescence and Western blotting to analysis the distribution of Nm23H1 in 95D cells treated with serum after serum starvation for 24 hours. Results: Serum stimulation ould induced lamellipodia formation in 95D cells. Most of Nm23H1 existed in cytosol, but there was significant Nm23H1 staining in new formed lamellipodia. Using subcellular fractionation and Western blotting, we confirmed that Nm23H1 could be translocated to membrane after serum stimulation, which happened rapidly, peaked at about 30 minutes, and slightly reduced to above basal level at 8 hours. Conclusions: Our results indicated that Nm23H1 transloction is associated with the formation of lamellipodia, which may provide a clue to further elucidate the role of Nm23H1 played in tumor suppression.",

keywords = "Lamellipodia, Membrane translocation, Nm23H1",

author = "Li Tan and Ping Li and Sun, {Xin Hui} and Li, {Chun Yang} and Chang Tong and Jin Fan and Zhu, {Yun Song}",

year = "2005",

month = may,

language = "English (US)",

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pages = "255--258",

journal = "Fudan University Journal of Medical Sciences",

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publisher = "Fudan University",

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T1 - Serum-induced membrane translocation of Nm23H1 in 95D cells

AU - Tan, Li

AU - Li, Ping

AU - Sun, Xin Hui

AU - Li, Chun Yang

AU - Tong, Chang

AU - Fan, Jin

AU - Zhu, Yun Song

PY - 2005/5

Y1 - 2005/5

N2 - Purpose: To explore the cellular distribution of Nm23H1 in human giant-cell lung carcinoma 95D cells. Methods: We used Immunofluorescence and Western blotting to analysis the distribution of Nm23H1 in 95D cells treated with serum after serum starvation for 24 hours. Results: Serum stimulation ould induced lamellipodia formation in 95D cells. Most of Nm23H1 existed in cytosol, but there was significant Nm23H1 staining in new formed lamellipodia. Using subcellular fractionation and Western blotting, we confirmed that Nm23H1 could be translocated to membrane after serum stimulation, which happened rapidly, peaked at about 30 minutes, and slightly reduced to above basal level at 8 hours. Conclusions: Our results indicated that Nm23H1 transloction is associated with the formation of lamellipodia, which may provide a clue to further elucidate the role of Nm23H1 played in tumor suppression.

AB - Purpose: To explore the cellular distribution of Nm23H1 in human giant-cell lung carcinoma 95D cells. Methods: We used Immunofluorescence and Western blotting to analysis the distribution of Nm23H1 in 95D cells treated with serum after serum starvation for 24 hours. Results: Serum stimulation ould induced lamellipodia formation in 95D cells. Most of Nm23H1 existed in cytosol, but there was significant Nm23H1 staining in new formed lamellipodia. Using subcellular fractionation and Western blotting, we confirmed that Nm23H1 could be translocated to membrane after serum stimulation, which happened rapidly, peaked at about 30 minutes, and slightly reduced to above basal level at 8 hours. Conclusions: Our results indicated that Nm23H1 transloction is associated with the formation of lamellipodia, which may provide a clue to further elucidate the role of Nm23H1 played in tumor suppression.

KW - Lamellipodia

KW - Membrane translocation

KW - Nm23H1

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