Shot-gun sequencing strategy for long-range genome mapping: A pilot study

Eugene R. Zabarovsky, Vladimir I. Kashuba, Bertil Pettersson, Nikolai Petrov, Vladimir Zakharyev, Rinat Gizatullin, Tatyana Lebedeva, George Klein, Ekaterina S. Pokrovskaya, Veronika I. Zabarovska, Rando Allikmets, Rikard Erlandsson, Dmitry Domninsky, Janos Sumegi, Eric J. Stanbridge, Gösta Winberg, Mathias Uhlen

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

We have recently proposed a strategy for construction of long-range physical maps based on random sequencing of NotI linking and jumping clones. Here, we present results of sequence comparison between 168 NotI linking (100 of them were sequenced from both sides) and 81 chromosome 3-specific jumping clones. We were able to identify 14 NotI jumping clones (17%), each joined with two NotI linking clones. The average size of chromosomal jumps was about 650 kb. The assembled 42 NotI genomic fragments correspond to 12-15% of chromosome 3. These results demonstrate the value of random sequencing of NotI linking and jumping clones for genome mapping. This mapping proposal can be used for connecting physical and genetic maps of the human genome and will be a valuable supplement to YAC and cosmid library based mapping projects.

Original languageEnglish (US)
Pages (from-to)495-500
Number of pages6
JournalGenomics
Volume21
Issue number3
DOIs
StatePublished - Jun 1994

ASJC Scopus subject areas

  • Genetics

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    Zabarovsky, E. R., Kashuba, V. I., Pettersson, B., Petrov, N., Zakharyev, V., Gizatullin, R., Lebedeva, T., Klein, G., Pokrovskaya, E. S., Zabarovska, V. I., Allikmets, R., Erlandsson, R., Domninsky, D., Sumegi, J., Stanbridge, E. J., Winberg, G., & Uhlen, M. (1994). Shot-gun sequencing strategy for long-range genome mapping: A pilot study. Genomics, 21(3), 495-500. https://doi.org/10.1006/geno.1994.1307