TY - JOUR
T1 - Shot-gun sequencing strategy for long-range genome mapping
T2 - A pilot study
AU - Zabarovsky, Eugene R.
AU - Kashuba, Vladimir I.
AU - Pettersson, Bertil
AU - Petrov, Nikolai
AU - Zakharyev, Vladimir
AU - Gizatullin, Rinat
AU - Lebedeva, Tatyana
AU - Klein, George
AU - Pokrovskaya, Ekaterina S.
AU - Zabarovska, Veronika I.
AU - Allikmets, Rando
AU - Erlandsson, Rikard
AU - Domninsky, Dmitry
AU - Sumegi, Janos
AU - Stanbridge, Eric J.
AU - Winberg, Gösta
AU - Uhlen, Mathias
PY - 1994/6
Y1 - 1994/6
N2 - We have recently proposed a strategy for construction of long-range physical maps based on random sequencing of NotI linking and jumping clones. Here, we present results of sequence comparison between 168 NotI linking (100 of them were sequenced from both sides) and 81 chromosome 3-specific jumping clones. We were able to identify 14 NotI jumping clones (17%), each joined with two NotI linking clones. The average size of chromosomal jumps was about 650 kb. The assembled 42 NotI genomic fragments correspond to 12-15% of chromosome 3. These results demonstrate the value of random sequencing of NotI linking and jumping clones for genome mapping. This mapping proposal can be used for connecting physical and genetic maps of the human genome and will be a valuable supplement to YAC and cosmid library based mapping projects.
AB - We have recently proposed a strategy for construction of long-range physical maps based on random sequencing of NotI linking and jumping clones. Here, we present results of sequence comparison between 168 NotI linking (100 of them were sequenced from both sides) and 81 chromosome 3-specific jumping clones. We were able to identify 14 NotI jumping clones (17%), each joined with two NotI linking clones. The average size of chromosomal jumps was about 650 kb. The assembled 42 NotI genomic fragments correspond to 12-15% of chromosome 3. These results demonstrate the value of random sequencing of NotI linking and jumping clones for genome mapping. This mapping proposal can be used for connecting physical and genetic maps of the human genome and will be a valuable supplement to YAC and cosmid library based mapping projects.
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U2 - 10.1006/geno.1994.1307
DO - 10.1006/geno.1994.1307
M3 - Article
C2 - 7959725
AN - SCOPUS:0028339338
SN - 0888-7543
VL - 21
SP - 495
EP - 500
JO - Genomics
JF - Genomics
IS - 3
ER -