Shotgun proteomic analysis of human-induced sputum

Ben Nicholas, Paul Skipp, Richard Mould, Stephen Rennard, Donna E. Davies, C. David O'Connor, Ratko Djukanović

Research output: Contribution to journalArticle

87 Scopus citations

Abstract

Induced sputum is a readily accessible biological fluid whose composition may alter as a consequence of disease. To date, however, the proteins that routinely populate this biofluid are largely unknown, in part due to the technical difficulties in processing such mucin-rich samples. To provide a catalogue of sputum proteins, we have surveyed the proteome of human-induced sputum (sputome). A combination of 2-D gel analysis and GeLC-MS/MS allowed a total of 191 human proteins to be confidently assigned. In addition to the expected components, several hitherto unreported proteins were found to be present, including three members of the annexin family, kallikreins 1 and 11, and peroxiredoxins 1, 2 and 5. Other sets of proteins identified included four proteins previously annotated as hypothetical or conserved hypothetical. Taken together, these data represent the first extensive survey of the proteome of induced sputum and provide a platform for future identification of biomarkers of lung disease.

Original languageEnglish (US)
Pages (from-to)4390-4401
Number of pages12
JournalProteomics
Volume6
Issue number15
DOIs
StatePublished - Aug 2006
Externally publishedYes

Keywords

  • 2-D PAGE
  • GeLC-MS/MS
  • Human bronchoalveolar lavage fluid proteins
  • Induced sputum

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Nicholas, B., Skipp, P., Mould, R., Rennard, S., Davies, D. E., O'Connor, C. D., & Djukanović, R. (2006). Shotgun proteomic analysis of human-induced sputum. Proteomics, 6(15), 4390-4401. https://doi.org/10.1002/pmic.200600011