Shotgun sphingolipid analysis of human aqueous humor

Anna Trzeciecka, Ulises Arbelo, Arturo Barron, Genea Edwards, Sruthi Sampathkumar, Carol Toris, Sanjoy K. Bhattacharya

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

This protocol provides a step-by-step guide to shotgun sphingolipid analysis of aqueous humor. We describe the Bligh and Dyer crude lipid extraction method and electrospray ionization tandem mass spectrometry (ESI-MS/MS) coupled with MZmine 2.21 data processing for identification and ratiometric quantitation of sphingosine, sphingosine-1-phosphate, sphingomyelin, and ceramide.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages97-107
Number of pages11
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1695
ISSN (Print)1064-3745

Keywords

  • Aqueous humor
  • Ceramide
  • Electrospray ionization-tandem mass spectrometry (ESI-MS/MS)
  • MZmine 2.21
  • Shotgun lipidomics
  • Sphingolipids
  • Sphingomyelin
  • Sphingosine
  • Sphingosine-1-phosphate

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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