Simplified DGS procedure for large-scale genome structural study

Yong Chul Jung, Jia Xu, Jun Chen, Yeong C. Kim, David J. Winchester, San Ming Wang

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Ditag genome scanning (DGS) uses next-generation DNA sequencing to sequence the ends of ditag fragments produced by restriction enzymes. These sequences are compared to known genome sequences to determine their structure. In order to use DGS for large-scale genome structural studies, we have substantially revised the original protocol by replacing the in vivo genomic DNA cloning with in vitro adaptor ligation, eliminating the ditag concatemerization steps, and replacing the 454 sequencer with Solexa or SOLiD sequencers for ditag sequence collection. This revised protocol further increases genome coverage and resolution and allows DGS to be used to analyze multiple genomes simultaneously.

Original languageEnglish (US)
Pages (from-to)969-971
Number of pages3
Issue number5
StatePublished - Nov 2009
Externally publishedYes


  • Ditag
  • Genome structure
  • Next-generation DNA sequencer
  • Paired-end
  • Restriction fragment

ASJC Scopus subject areas

  • Biotechnology
  • General Biochemistry, Genetics and Molecular Biology


Dive into the research topics of 'Simplified DGS procedure for large-scale genome structural study'. Together they form a unique fingerprint.

Cite this