Simplified DGS procedure for large-scale genome structural study

Yong Chul Jung, Jia Xu, Jun Chen, Yeong C. Kim, David J. Winchester, San Ming Wang

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Ditag genome scanning (DGS) uses next-generation DNA sequencing to sequence the ends of ditag fragments produced by restriction enzymes. These sequences are compared to known genome sequences to determine their structure. In order to use DGS for large-scale genome structural studies, we have substantially revised the original protocol by replacing the in vivo genomic DNA cloning with in vitro adaptor ligation, eliminating the ditag concatemerization steps, and replacing the 454 sequencer with Solexa or SOLiD sequencers for ditag sequence collection. This revised protocol further increases genome coverage and resolution and allows DGS to be used to analyze multiple genomes simultaneously.

Original languageEnglish (US)
Pages (from-to)969-971
Number of pages3
JournalBioTechniques
Volume47
Issue number5
DOIs
StatePublished - Nov 2009

Keywords

  • Ditag
  • Genome structure
  • Next-generation DNA sequencer
  • Paired-end
  • Restriction fragment

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Jung, Y. C., Xu, J., Chen, J., Kim, Y. C., Winchester, D. J., & Wang, S. M. (2009). Simplified DGS procedure for large-scale genome structural study. BioTechniques, 47(5), 969-971. https://doi.org/10.2144/000113294