@article{a687ce50905d45bb866ad2395834c848,
title = "Simultaneous Delivery of Multiple Antimicrobial Agents by Biphasic Scaffolds for Effective Treatment of Wound Biofilms",
abstract = "Biofilms pose a major challenge to control wound-associated infections. Due to biofilm impenetrability, traditional antimicrobial agents are often ineffective in combating biofilms. Herein, a biphasic scaffold is reported as an antimicrobial delivery system by integrating nanofiber mats with dissolvable microneedle arrays for the effective treatment of bacterial biofilms. Different combinations of antimicrobial agents, including AgNO3, Ga(NO3)3, and vancomycin, are incorporated into nanofiber mats by coaxial electrospinning, which enables sustained delivery of these drugs. The antimicrobial agents-incorporated dissolvable microneedle arrays allow direct penetration of drugs into biofilms. By optimizing the administration strategies, drug combinations, and microneedle densities, biphasic scaffolds are able to eradicate both methicillin-resistant Staphylococcus aureus (MRSA) and MRSA/Pseudomonas aeruginosa blend biofilms in an ex vivo human skin wound infection model without necessitating surgical debridement. Taken together, the combinatorial system comprises of nanofiber mats and microneedle arrays can provide an efficacious delivery of multiple antimicrobial agents for the treatment of bacterial biofilms in wounds.",
keywords = "antimicrobial agents, biofilms, microneedle arrays, nanofiber mats, wounds",
author = "Yajuan Su and Alec McCarthy and Wong, {Shannon L.} and Hollins, {Ronald R.} and Guangshun Wang and Jingwei Xie",
note = "Funding Information: The human skin tissues were collected from patients who underwent plastic surgery, who gave their informed, written consent, and the IRB protocol was approved by the University of Nebraska Medical Center (Protocol #152-14-EP). This work was supported in part by startup funds from the University of Nebraska Medical Center (UNMC), National Institute of General Medical Science (NIGMS) of the National Institutes of Health under Award No. R01GM123081 to J.X. and R01GM138552 to J.X. and G.W., UNMC Regenerative Medicine Program pilot grant, and Nebraska Research Initiative grant, and NE LB606. Funding Information: The human skin tissues were collected from patients who underwent plastic surgery, who gave their informed, written consent, and the IRB protocol was approved by the University of Nebraska Medical Center (Protocol #152‐14‐EP). This work was supported in part by startup funds from the University of Nebraska Medical Center (UNMC), National Institute of General Medical Science (NIGMS) of the National Institutes of Health under Award No. R01GM123081 to J.X. and R01GM138552 to J.X. and G.W., UNMC Regenerative Medicine Program pilot grant, and Nebraska Research Initiative grant, and NE LB606. Publisher Copyright: {\textcopyright} 2021 Wiley-VCH GmbH",
year = "2021",
month = jun,
day = "23",
doi = "10.1002/adhm.202100135",
language = "English (US)",
volume = "10",
journal = "Advanced healthcare materials",
issn = "2192-2640",
publisher = "John Wiley and Sons Ltd",
number = "12",
}