TY - JOUR
T1 - Simultaneous determination of acetamiprid and 6-chloronicotinic acid in environmental samples by using ion chromatography hyphenated to online photoinduced fluorescence detector
AU - Subhani, Qamar
AU - Muhammad, Nadeem
AU - Huang, Zhouman
AU - Asif, Muhammad
AU - Hussain, Irshad
AU - Zahid, Muhammad
AU - Hairong, Cui
AU - Zhu, Yan
AU - Guo, Dandan
N1 - Funding Information:
This work is funded by the Zhejiang Provincial Natural Science Foundation of China (Grant No. LZ16B050001).
Publisher Copyright:
© 2020 Wiley-VCH GmbH
PY - 2020/10/1
Y1 - 2020/10/1
N2 - This study aims to introduce a simple, sensitive, and cost-effective method for the simultaneous determination of acetamiprid and its main metabolite 6-chloronicotinic acid in environmental samples by using a nonsuppressed ion chromatography hyphenated with an online postcolumn photoinduced fluorescence detection system. The fluorescence detector wavelengths λex/λem = 257/382 nm was set for up to 6.0 min for acetamiprid, while λex/λem = 231/370 nm programmed for 6-chloronicotinic acid for the rest of the analysis time. Both samples were treated by applying miniaturized quick, easy, cheap, effective, rugged, and safe method before the separation of analytes on an IonPac® AS11-HC column by pumping 40 mM NaOH having minuscule content of acetonitrile (5%, v/v) as an eluent. Both intrinsically nonfluorescent analytes were turned-on by online postcolumn photoinduced derivatization, avoiding the need for complex chemical derivatization or addition of a postcolumn extra pump. The developed method was appraised for the analysis of environmental samples, exhibiting excellent linearity (0.050-10 μg/mL) with a correlation coefficient greater than 0.9993 for both analytes. Whereas, obtained limit of detection (0.025-0.0072 μg/mL), recoveries (98.02-116.00%), and inter- and intraday precision (≤3.02 %) were satisfactory for both compounds in environmental samples.
AB - This study aims to introduce a simple, sensitive, and cost-effective method for the simultaneous determination of acetamiprid and its main metabolite 6-chloronicotinic acid in environmental samples by using a nonsuppressed ion chromatography hyphenated with an online postcolumn photoinduced fluorescence detection system. The fluorescence detector wavelengths λex/λem = 257/382 nm was set for up to 6.0 min for acetamiprid, while λex/λem = 231/370 nm programmed for 6-chloronicotinic acid for the rest of the analysis time. Both samples were treated by applying miniaturized quick, easy, cheap, effective, rugged, and safe method before the separation of analytes on an IonPac® AS11-HC column by pumping 40 mM NaOH having minuscule content of acetonitrile (5%, v/v) as an eluent. Both intrinsically nonfluorescent analytes were turned-on by online postcolumn photoinduced derivatization, avoiding the need for complex chemical derivatization or addition of a postcolumn extra pump. The developed method was appraised for the analysis of environmental samples, exhibiting excellent linearity (0.050-10 μg/mL) with a correlation coefficient greater than 0.9993 for both analytes. Whereas, obtained limit of detection (0.025-0.0072 μg/mL), recoveries (98.02-116.00%), and inter- and intraday precision (≤3.02 %) were satisfactory for both compounds in environmental samples.
KW - acetamiprid
KW - fluorescence
KW - ion chromatography
KW - neonicotinoids
KW - online
KW - postcolumn
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U2 - 10.1002/jssc.202000635
DO - 10.1002/jssc.202000635
M3 - Article
C2 - 32844548
AN - SCOPUS:85091350586
SN - 1615-9306
VL - 43
SP - 3921
EP - 3930
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 20
ER -