Simultaneous determination of acetamiprid and 6-chloronicotinic acid in environmental samples by using ion chromatography hyphenated to online photoinduced fluorescence detector

Qamar Subhani, Nadeem Muhammad, Zhouman Huang, Muhammad Asif, Irshad Hussain, Muhammad Zahid, Cui Hairong, Yan Zhu, Dandan Guo

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

This study aims to introduce a simple, sensitive, and cost-effective method for the simultaneous determination of acetamiprid and its main metabolite 6-chloronicotinic acid in environmental samples by using a nonsuppressed ion chromatography hyphenated with an online postcolumn photoinduced fluorescence detection system. The fluorescence detector wavelengths λexem = 257/382 nm was set for up to 6.0 min for acetamiprid, while λexem = 231/370 nm programmed for 6-chloronicotinic acid for the rest of the analysis time. Both samples were treated by applying miniaturized quick, easy, cheap, effective, rugged, and safe method before the separation of analytes on an IonPac® AS11-HC column by pumping 40 mM NaOH having minuscule content of acetonitrile (5%, v/v) as an eluent. Both intrinsically nonfluorescent analytes were turned-on by online postcolumn photoinduced derivatization, avoiding the need for complex chemical derivatization or addition of a postcolumn extra pump. The developed method was appraised for the analysis of environmental samples, exhibiting excellent linearity (0.050-10 μg/mL) with a correlation coefficient greater than 0.9993 for both analytes. Whereas, obtained limit of detection (0.025-0.0072 μg/mL), recoveries (98.02-116.00%), and inter- and intraday precision (≤3.02 %) were satisfactory for both compounds in environmental samples.

Original languageEnglish (US)
Pages (from-to)3921-3930
Number of pages10
JournalJournal of Separation Science
Volume43
Issue number20
DOIs
StatePublished - Oct 1 2020

Keywords

  • acetamiprid
  • fluorescence
  • ion chromatography
  • neonicotinoids
  • online
  • postcolumn

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

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