Simultaneous determination of zidovudine and lamivudine from rat plasma, amniotic fluid and tissues by HPLC

Yazen Alnouti, Catherine A. White, Michael G. Bartlett

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

A sensitive HPLC method has been developed and validated for the simultaneous quantification of zidovudine (AZT) and lamivudine (3TC) in rat plasma, amniotic fluid and placental and fetal tissues. Samples were processed by solid-phase extraction using C2 cartridges. Chromatography was performed using a phenyl column (5 μm, 150 × 2 mm i.d.) under a flow rate of 0.2 mL/min. The mobile phase consisted of 8% acetonitrile in 5 mm 1-heptane sulfonic acid dissolved in 30 mm ammonium formate buffer (pH 3.3). The method was validated in the range 0.25-50 μg/mL for both 3TC and AZT in the four biological matrices. Finally, the method was applied to a study involving fetal transport of co-administration of these compounds in a pregnant rat.

Original languageEnglish (US)
Pages (from-to)641-647
Number of pages7
JournalBiomedical Chromatography
Volume18
Issue number9
DOIs
StatePublished - Nov 2004

Keywords

  • Bioanalytical
  • Ion-pairing chromatography
  • Lamivudine
  • Validation
  • Zidovudine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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