Simultaneous measurement of glucose and glutamine in insect cell culture media by near infrared spectroscopy

Mark R. Riley, Martin Rhiel, Xiangji Zhou, Mark A. Arnold, David W. Murhammer

Research output: Contribution to journalArticle

83 Scopus citations

Abstract

Noninvasive techniques were developed to monitor the glucose and glutamine concentration of a cell culture media in insect cell bioreactors. The measured standard error of prediction (SEP) and mean percent error (MPE) of glucose and glutamine were low considering the changing complexity of the growth media due to the shifting levels of amino acids, carbohydrates, yeastolate, proteins, and cell debris.

Original languageEnglish (US)
Pages (from-to)11-15
Number of pages5
JournalBiotechnology and Bioengineering
Volume55
Issue number1
DOIs
StatePublished - Jul 5 1997

Keywords

  • Bioreactor monitoring
  • Insect cell culture
  • NIR spectroscopy

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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