Simultaneous measurement of glucose and glutamine in insect cell culture media by near infrared spectroscopy

Mark R. Riley; Martin Rhiel; Xiangji Zhou; Mark A. Arnold; David W. Murhammer

doi:10.1002/(sici)1097-0290(19970705)55:1<11::aid-bit2>3.0.co;2-%23

Simultaneous measurement of glucose and glutamine in insect cell culture media by near infrared spectroscopy

Mark R. Riley, Martin Rhiel, Xiangji Zhou, Mark A. Arnold, David W. Murhammer

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Noninvasive techniques were developed to monitor the glucose and glutamine concentration of a cell culture media in insect cell bioreactors. The measured standard error of prediction (SEP) and mean percent error (MPE) of glucose and glutamine were low considering the changing complexity of the growth media due to the shifting levels of amino acids, carbohydrates, yeastolate, proteins, and cell debris.

Original language	English (US)
Pages (from-to)	11-15
Number of pages	5
Journal	Biotechnology and Bioengineering
Volume	55
Issue number	1
DOIs	https://doi.org/10.1002/(sici)1097-0290(19970705)55:1<11::aid-bit2>3.0.co;2-%23
State	Published - Jul 5 1997
Externally published	Yes

Keywords

Bioreactor monitoring
Insect cell culture
NIR spectroscopy

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1002/(sici)1097-0290(19970705)55:1<11::aid-bit2>3.0.co;2-%23

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abstract = "Noninvasive techniques were developed to monitor the glucose and glutamine concentration of a cell culture media in insect cell bioreactors. The measured standard error of prediction (SEP) and mean percent error (MPE) of glucose and glutamine were low considering the changing complexity of the growth media due to the shifting levels of amino acids, carbohydrates, yeastolate, proteins, and cell debris.",

keywords = "Bioreactor monitoring, Insect cell culture, NIR spectroscopy",

author = "Riley, {Mark R.} and Martin Rhiel and Xiangji Zhou and Arnold, {Mark A.} and Murhammer, {David W.}",

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AU - Rhiel, Martin

AU - Zhou, Xiangji

AU - Arnold, Mark A.

AU - Murhammer, David W.

PY - 1997/7/5

Y1 - 1997/7/5

N2 - Noninvasive techniques were developed to monitor the glucose and glutamine concentration of a cell culture media in insect cell bioreactors. The measured standard error of prediction (SEP) and mean percent error (MPE) of glucose and glutamine were low considering the changing complexity of the growth media due to the shifting levels of amino acids, carbohydrates, yeastolate, proteins, and cell debris.

AB - Noninvasive techniques were developed to monitor the glucose and glutamine concentration of a cell culture media in insect cell bioreactors. The measured standard error of prediction (SEP) and mean percent error (MPE) of glucose and glutamine were low considering the changing complexity of the growth media due to the shifting levels of amino acids, carbohydrates, yeastolate, proteins, and cell debris.

KW - Bioreactor monitoring

KW - Insect cell culture

KW - NIR spectroscopy

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Simultaneous measurement of glucose and glutamine in insect cell culture media by near infrared spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

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