TY - JOUR
T1 - Single immunization with a suboptimal antigen dose encapsulated into polyanhydride microparticles promotes high titer and avid antibody responses
AU - Huntimer, Lucas
AU - Wilson Welder, Jennifer H.
AU - Ross, Kathleen
AU - Carrillo-Conde, Brenda
AU - Pruisner, Lynn
AU - Wang, Chong
AU - Narasimhan, Balaji
AU - Wannemuehler, Michael J.
AU - Ramer-Tait, Amanda E.
PY - 2013/1
Y1 - 2013/1
N2 - Microparticle adjuvants based on biodegradable polyanhydrides were used to provide controlled delivery of a model antigen, ovalbumin (Ova), to mice. Ova was encapsulated into two different polyanhydride microparticle formulations to evaluate the influence of polymer chemistry on the nature and magnitude of the humoral immune response after administration of a suboptimal dose. Subcutaneous administration of a single dose of polyanhydride microparticles containing 25 μg of Ova elicited humoral immune responses that were comparable in magnitude to that induced by soluble doses of 400-1600 μg Ova. In contrast, the avidity of the Ova-specific antibodies was greater in mice administered the microparticle formulations in comparison to the higher soluble doses. Finally, the microparticle delivery system primed an anamnestic immune response as evidenced by the significant increases in Ova-specific antibody when mice were administered an antigenic challenge of 25 μg of Ova at 12 weeks post-vaccination. Together, these results indicate that encapsulation of antigens into polyanhydride microparticles facilitates isotype switching, establishes immunologic memory, and the humoral response was characterized by a higher quality antibody response.
AB - Microparticle adjuvants based on biodegradable polyanhydrides were used to provide controlled delivery of a model antigen, ovalbumin (Ova), to mice. Ova was encapsulated into two different polyanhydride microparticle formulations to evaluate the influence of polymer chemistry on the nature and magnitude of the humoral immune response after administration of a suboptimal dose. Subcutaneous administration of a single dose of polyanhydride microparticles containing 25 μg of Ova elicited humoral immune responses that were comparable in magnitude to that induced by soluble doses of 400-1600 μg Ova. In contrast, the avidity of the Ova-specific antibodies was greater in mice administered the microparticle formulations in comparison to the higher soluble doses. Finally, the microparticle delivery system primed an anamnestic immune response as evidenced by the significant increases in Ova-specific antibody when mice were administered an antigenic challenge of 25 μg of Ova at 12 weeks post-vaccination. Together, these results indicate that encapsulation of antigens into polyanhydride microparticles facilitates isotype switching, establishes immunologic memory, and the humoral response was characterized by a higher quality antibody response.
KW - adjuvant
KW - avidity
KW - microparticle
KW - ovalbumin
KW - polyanhydride
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U2 - 10.1002/jbm.b.32820
DO - 10.1002/jbm.b.32820
M3 - Article
C2 - 23143744
AN - SCOPUS:84871389613
SN - 1552-4973
VL - 101 B
SP - 91
EP - 98
JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials
JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials
IS - 1
ER -