SiRNA-directed DNA methylation in plants

Meng Xie, Bin Yu

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


DNA cytosine methylationis an important epigenetic process that is correlated with transgene silencing, transposon suppression, and gene imprinting. In plants, small interfering RNAs (siRNAs) can trigger DNA methylation at loci containing their homolog sequences through a process called RNA-directed DNA methylation (RdDM). In canonical RdDM, 24 nucleotide (nt) siRNAs (ra-siRNAs) will be loaded into their effector protein called ARGONAUTE 4 (AGO4) and subsequently targeted to RdDM loci through base-pairing with the non-coding transcripts produced by DNA-directed RNA Polymerase V. Then, the AGO4-ra-siRNA will recruit the DNA methyltransferase to catalyze de novo DNA methylation. Recent studies also identified non-canonical RdDM pathways that involve microRNAs or 21 nt siRNAs. These RdDM pathways are biologically important since they control responses biotic and abiotic stresses, maintain genome stability and regulate development. Here, we summarize recent progresses of mechanisms governing canonical and noncanonical RdDM pathways.

Original languageEnglish (US)
Pages (from-to)23-31
Number of pages9
JournalCurrent Genomics
Issue number1
StatePublished - Jan 1 2015


  • DNA methylation
  • Plants
  • SiRNA

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'SiRNA-directed DNA methylation in plants'. Together they form a unique fingerprint.

Cite this