Site-specific delivery of oligonucleotides to hepatocytes after systemic administration

Lin Zhu, Zhaoyang Ye, Kun Cheng, Duane D. Miller, Ram I. Mahato

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


We previously complexed ODN with galactosylated poly(L-lysine) (Gal-PLL) to enhance its site-specific delivery to hepatocytes. To avoid the use of polycations, in this study we conjugated galactosylated poly(ethylene glycol) (Gal-PEG (MW of PEG: 3486 ± 500 Da)) to ODN via an acid-labile ester linkage of β-thiopropionate. Following tail vein injection into rats, Gal-PEG-33P-ODN rapidly cleared from the circulation and 60.2% of the injected dose accumulated in the liver at 30 min postinjection, which was significantly higher than that deposited after injection of 33P-ODNs. The plasma concentration versus time profile of Gal-PEG-33P-ODN was biphasic, with 4.38 ± 0.36 min as t1/2 of distribution and 118.61 ± 22.06 min as t1/2 of elimination. Prior administration of excess Gal-BSA decreased the hepatic uptake of Gal-PEG- 33P-ODN from 60.2% to 35.9%, suggesting galactose triggers the asialoglycoprotein receptor-mediated endocytosis of Gal-PEG-33P-ODN by hepatocytes. A large proportion of the injected Gal-PEG-33P-ODN was taken up by the hepatocytes as evidenced by determination of radioactivity in the digested liver cells upon liver perfusion and separation by centrifugation on a Nycodenz gradient. In conclusion, Gal-PEG-ODN conjugate may be used for treating a variety of liver diseases.

Original languageEnglish (US)
Pages (from-to)290-298
Number of pages9
JournalBioconjugate Chemistry
Issue number1
StatePublished - Jan 2008

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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