Site-specific incorporation of methyl- and acetyl-lysine analogues into recombinant proteins

Jiantao Guo, Jiangyun Wang, Jong Seok Lee, Peter G. Schultz

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

(Chemical Equation Presented) Location, location, location: A new chemoselective method to site- and degree-specifically install lysine analogues into recombinant proteins provides facile access to near-homogeneously methylated and acetylated histone analogues (see scheme; R1, R 2, R3 = H, Me, acetyl), which can be used to study the impact of histone modification on chromatin structure and function.

Original languageEnglish (US)
Pages (from-to)6399-6401
Number of pages3
JournalAngewandte Chemie - International Edition
Volume47
Issue number34
DOIs
StatePublished - Aug 11 2008

Keywords

  • Dehydroalanine
  • Histones
  • Lysine derivatives
  • Protein modifications

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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