Size-dependent Catalysis of Chlorovirus Population Growth by A Messy Feeding Predator

John P. DeLong, Zeina Al-Ameeli, Shelby Lyon, James L. Van Etten, David D. Dunigan

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Many chloroviruses replicate in endosymbiotic zoochlorellae that are protected from infection by their symbiotic host. To reach the high virus concentrations that often occur in natural systems, a mechanism is needed to release zoochlorellae from their hosts. We demonstrate that the ciliate predator Didinium nasutum foraging on zoochlorellae-bearing Paramecium bursaria can release live zoochlorellae from the ruptured prey cell that can then be infected by chloroviruses. The catalysis process is very effective, yielding roughly 95% of the theoretical infectious virus yield as determined by sonication of P. bursaria. Chlorovirus activation is more effective with smaller Didinia, as larger Didinia typically consume entire P. bursaria cells without rupturing them, precluding the release of zoochlorellae. We also show that the timing of Chlorovirus growth is tightly linked to the predator-prey cycle between Didinium and Paramecium, with the most rapid increase in chloroviruses temporally linked to the peak foraging rate of Didinium, supporting the idea that predator-prey cycles can drive cycles of Chlorovirus abundance.

Original languageEnglish (US)
Pages (from-to)847-853
Number of pages7
JournalMicrobial Ecology
Volume75
Issue number4
DOIs
StatePublished - May 1 2018

Keywords

  • Chloroviruses
  • Didinium
  • Paramecium bursaria
  • Predator-prey interaction
  • Trait mediated
  • Virus dynamics

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology
  • Soil Science

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