SMA1, a homolog of the splicing factor Prp28, has a multifaceted role in miRNA biogenesis in Arabidopsis

Shengjun Li; Ran Xu; Aixia Li; Kan Liu; Liqing Gu; Mu Li; Hairui Zhang; Yueying Zhang; Shangshang Zhuang; Quanhui Wang; Gang Gao; Na Li; Chi Zhang; Yunhai Li; Bin Yu

doi:10.1093/nar/gky591

SMA1, a homolog of the splicing factor Prp28, has a multifaceted role in miRNA biogenesis in Arabidopsis

Shengjun Li, Ran Xu, Aixia Li, Kan Liu, Liqing Gu, Mu Li, Hairui Zhang, Yueying Zhang, Shangshang Zhuang, Quanhui Wang, Gang Gao, Na Li, Chi Zhang, Yunhai Li, Bin Yu

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress gene expression. In plants, the RNase III enzyme Dicer-like (DCL1) processes primary miRNAs (pri-miRNAs) into miRNAs. Here, we show that SMALL1 (SMA1), a homolog of the DEAD-box pre-mRNA splicing factor Prp28, plays essential roles in miRNA biogenesis in Arabidopsis. A hypomorphic sma1-1 mutation causes growth defects and reduces miRNA accumulation correlated with increased target transcript levels. SMA1 interacts with the DCL1 complex and positively influences pri-miRNA processing. Moreover, SMA1 binds the promoter region of genes encoding pri-miRNAs (MIRs) and is required for MIR transcription. Furthermore, SMA1 also enhances the abundance of the DCL1 protein levels through promoting the splicing of the DCL1 pre-mRNAs. Collectively, our data provide new insights into the function of SMA1/Prp28 in regulating miRNA abundance in plants.

Original language	English (US)
Pages (from-to)	9148-9159
Number of pages	12
Journal	Nucleic acids research
Volume	46
Issue number	17
DOIs	https://doi.org/10.1093/nar/gky591
State	Published - Sep 28 2018

ASJC Scopus subject areas

Genetics

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@article{084da118dc60409593be00308fa3dd49,

title = "SMA1, a homolog of the splicing factor Prp28, has a multifaceted role in miRNA biogenesis in Arabidopsis",

abstract = "MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress gene expression. In plants, the RNase III enzyme Dicer-like (DCL1) processes primary miRNAs (pri-miRNAs) into miRNAs. Here, we show that SMALL1 (SMA1), a homolog of the DEAD-box pre-mRNA splicing factor Prp28, plays essential roles in miRNA biogenesis in Arabidopsis. A hypomorphic sma1-1 mutation causes growth defects and reduces miRNA accumulation correlated with increased target transcript levels. SMA1 interacts with the DCL1 complex and positively influences pri-miRNA processing. Moreover, SMA1 binds the promoter region of genes encoding pri-miRNAs (MIRs) and is required for MIR transcription. Furthermore, SMA1 also enhances the abundance of the DCL1 protein levels through promoting the splicing of the DCL1 pre-mRNAs. Collectively, our data provide new insights into the function of SMA1/Prp28 in regulating miRNA abundance in plants.",

author = "Shengjun Li and Ran Xu and Aixia Li and Kan Liu and Liqing Gu and Mu Li and Hairui Zhang and Yueying Zhang and Shangshang Zhuang and Quanhui Wang and Gang Gao and Na Li and Chi Zhang and Yunhai Li and Bin Yu",

note = "Funding Information: Nebraska Soybean Board [1727 to B.Y.]; National Science Foundation [MCB1808182 to B.Y.]; National Natural Science Foundation of China [31425004, 91417304, 91017014 to Y.L.; 31400249 to R.X.]; Ministry of Agriculture of China [2016ZX08009-003 to N.L.]; Strategic Priority Research Program {\textquoteleft}Molecular Mechanism of Plant Growth and Development{\textquoteright} [XDBP401 to Y.L.]; Pioneer Hundred Program of Chinese Academy of Sciences (to S.L.). Funding for open access charge: Pioneer Hundred Program of Chinese Academy of Sciences. Conflict of interest statement. None declared. Publisher Copyright: {\textcopyright} The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.",

year = "2018",

month = sep,

day = "28",

doi = "10.1093/nar/gky591",

language = "English (US)",

volume = "46",

pages = "9148--9159",

journal = "Nucleic Acids Research",

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T1 - SMA1, a homolog of the splicing factor Prp28, has a multifaceted role in miRNA biogenesis in Arabidopsis

AU - Li, Shengjun

AU - Xu, Ran

AU - Li, Aixia

AU - Liu, Kan

AU - Gu, Liqing

AU - Li, Mu

AU - Zhang, Hairui

AU - Zhang, Yueying

AU - Zhuang, Shangshang

AU - Wang, Quanhui

AU - Gao, Gang

AU - Li, Na

AU - Zhang, Chi

AU - Li, Yunhai

AU - Yu, Bin

N1 - Funding Information: Nebraska Soybean Board [1727 to B.Y.]; National Science Foundation [MCB1808182 to B.Y.]; National Natural Science Foundation of China [31425004, 91417304, 91017014 to Y.L.; 31400249 to R.X.]; Ministry of Agriculture of China [2016ZX08009-003 to N.L.]; Strategic Priority Research Program ‘Molecular Mechanism of Plant Growth and Development’ [XDBP401 to Y.L.]; Pioneer Hundred Program of Chinese Academy of Sciences (to S.L.). Funding for open access charge: Pioneer Hundred Program of Chinese Academy of Sciences. Conflict of interest statement. None declared. Publisher Copyright: © The Author(s) 2018. Published by Oxford University Press on behalf of Nucleic Acids Research.

PY - 2018/9/28

Y1 - 2018/9/28

N2 - MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress gene expression. In plants, the RNase III enzyme Dicer-like (DCL1) processes primary miRNAs (pri-miRNAs) into miRNAs. Here, we show that SMALL1 (SMA1), a homolog of the DEAD-box pre-mRNA splicing factor Prp28, plays essential roles in miRNA biogenesis in Arabidopsis. A hypomorphic sma1-1 mutation causes growth defects and reduces miRNA accumulation correlated with increased target transcript levels. SMA1 interacts with the DCL1 complex and positively influences pri-miRNA processing. Moreover, SMA1 binds the promoter region of genes encoding pri-miRNAs (MIRs) and is required for MIR transcription. Furthermore, SMA1 also enhances the abundance of the DCL1 protein levels through promoting the splicing of the DCL1 pre-mRNAs. Collectively, our data provide new insights into the function of SMA1/Prp28 in regulating miRNA abundance in plants.

AB - MicroRNAs (miRNAs) are a class of small non-coding RNAs that repress gene expression. In plants, the RNase III enzyme Dicer-like (DCL1) processes primary miRNAs (pri-miRNAs) into miRNAs. Here, we show that SMALL1 (SMA1), a homolog of the DEAD-box pre-mRNA splicing factor Prp28, plays essential roles in miRNA biogenesis in Arabidopsis. A hypomorphic sma1-1 mutation causes growth defects and reduces miRNA accumulation correlated with increased target transcript levels. SMA1 interacts with the DCL1 complex and positively influences pri-miRNA processing. Moreover, SMA1 binds the promoter region of genes encoding pri-miRNAs (MIRs) and is required for MIR transcription. Furthermore, SMA1 also enhances the abundance of the DCL1 protein levels through promoting the splicing of the DCL1 pre-mRNAs. Collectively, our data provide new insights into the function of SMA1/Prp28 in regulating miRNA abundance in plants.

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U2 - 10.1093/nar/gky591

DO - 10.1093/nar/gky591

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C2 - 29982637

AN - SCOPUS:85054370816

SN - 0305-1048

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SP - 9148

EP - 9159

JO - Nucleic Acids Research

JF - Nucleic Acids Research

IS - 17

ER -

SMA1, a homolog of the splicing factor Prp28, has a multifaceted role in miRNA biogenesis in Arabidopsis

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