Sodium dodecyl sulfate hypersensitivity of clpP and clpB mutants of Escherichia coli

Soumitra Rajagopal, Narasimhan Sudarsan, Kenneth W. Nickerson

Research output: Contribution to journalArticle

28 Scopus citations

Abstract

We studied the hypersensitivity of clpP and clpB mutants of Escherichia coli to sodium dodecyl sulfate (SDS). Both wild-type E. coli MC4100 and Ion mutants grew in the presence of 10% SDS, whereas isogenic clpP and clpB single mutants could not grow above 0.5% SDS and clpA and clpX single mutants could not grow above 5.0% SDS. For wild-type E. coli, cellular ClpP levels as determined by Western immunoblot analysis increased ca. sixfold as the levels of added SDS increased from 0 to 2%. Capsular colanic acid, measured as uronic acid, increased ca. sixfold as the levels of added SDS increased from 2 to 10%. Based on these findings, 3 of the 19 previously identified SDS shock proteins (M. Adamowicz, P. M. Kelley, and K. W. Nickerson, J. Bacteriol. 173:229-233, 1991) are tentatively identified as ClpP, ClpX, and ClpB.

Original languageEnglish (US)
Pages (from-to)4117-4121
Number of pages5
JournalApplied and environmental microbiology
Volume68
Issue number8
DOIs
StatePublished - 2002

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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