TY - JOUR
T1 - Soft agar growth assays for transforming growth factors and mitogenic peptides
AU - Rizzino, Angie
N1 - Funding Information:
The author thanks Heather Rizzino for excellente ditorial assistance and for her many useful comments during the preparation of this chapter. Eric Ruff is also thanked for exceptional technicala ssistance and for helping in the assessmento f this chapter. This work was supported by grants from the Nebraska Department of Health (85-58) and the National Cancer Institute (CA 36727).
PY - 1987/1
Y1 - 1987/1
N2 - Many different tumor cells produce and release transforming growth factors (TGFs) that influence the growth and behavior of the cells that produce them. Consequently, the production of TGFs may have important effects on the growth of tumors in vivo. The ability to grow in soft agar is one of the best indicators of a cell's tumorigenic capacity. TGFs can induce the soft agar growth of nontransformed cells. TGFs are usually assayed by their ability to induce the soft agar growth of nontransformed cells. This chapter describes methods to examine TGFs and related growth factors for their ability to influence anchorage-independent growth. The chapter discusses the most commonly used indicator cell lines, the general methodology employed, the selection of appropriate culture media, and a rapid assay that is suitable for testing small numbers of cells for the release of factors that influence anchorage-independent growth.
AB - Many different tumor cells produce and release transforming growth factors (TGFs) that influence the growth and behavior of the cells that produce them. Consequently, the production of TGFs may have important effects on the growth of tumors in vivo. The ability to grow in soft agar is one of the best indicators of a cell's tumorigenic capacity. TGFs can induce the soft agar growth of nontransformed cells. TGFs are usually assayed by their ability to induce the soft agar growth of nontransformed cells. This chapter describes methods to examine TGFs and related growth factors for their ability to influence anchorage-independent growth. The chapter discusses the most commonly used indicator cell lines, the general methodology employed, the selection of appropriate culture media, and a rapid assay that is suitable for testing small numbers of cells for the release of factors that influence anchorage-independent growth.
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U2 - 10.1016/S0076-6879(87)46035-7
DO - 10.1016/S0076-6879(87)46035-7
M3 - Article
C2 - 3479675
AN - SCOPUS:0023507957
SN - 0076-6879
VL - 146
SP - 341
EP - 352
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -