Solid-phase combinatorial synthesis using MicroKan reactors, Rf tagging, and directed sorting

Xiao Yi Xiao, Rongshi Li, Hui Zhuang, Bill Ewing, K. Karunaratne, John Lillig, Rick Brown, K. C. Nicolaou

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

A modular system for high-output solid-phase combinatorial synthesis has been designed and developed. The system employs three technological innovations to achieve its high efficiency and reliability: (1) application of microreactors as the reaction units in solid-phase synthesis; (2) use of radiofrequency tagging as the non-chemical tracking method; and (3) development of the directed sorting technology for split and pool synthesis. The system has been successfully applied in the synthesis of compound libraries of several hundred to several thousand compounds in multi- milligrams per compound quantity by many organizations. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish (US)
Pages (from-to)44-50
Number of pages7
JournalBiotechnology and Bioengineering
Volume71
Issue number1
DOIs
StatePublished - Dec 2000

Keywords

  • Directed sorting
  • MicroKan reactor
  • Parallel synthesis
  • Radiofrequency tagging
  • Solid-phase synthesis
  • Split and pool

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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