Solid-phase combinatorial synthesis using MicroKan reactors, Rf tagging, and directed sorting

Xiao Yi Xiao; Rongshi Li; Hui Zhuang; Bill Ewing; K. Karunaratne; John Lillig; Rick Brown; K. C. Nicolaou

doi:10.1002/(SICI)1097-0290(200024)71:1<44::AID-BIT7>3.0.CO;2-J

Solid-phase combinatorial synthesis using MicroKan reactors, Rf tagging, and directed sorting

Xiao Yi Xiao, Rongshi Li, Hui Zhuang, Bill Ewing, K. Karunaratne, John Lillig, Rick Brown, K. C. Nicolaou

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

A modular system for high-output solid-phase combinatorial synthesis has been designed and developed. The system employs three technological innovations to achieve its high efficiency and reliability: (1) application of microreactors as the reaction units in solid-phase synthesis; (2) use of radiofrequency tagging as the non-chemical tracking method; and (3) development of the directed sorting technology for split and pool synthesis. The system has been successfully applied in the synthesis of compound libraries of several hundred to several thousand compounds in multi- milligrams per compound quantity by many organizations. (C) 2000 John Wiley and Sons, Inc.

Original language	English (US)
Pages (from-to)	44-50
Number of pages	7
Journal	Biotechnology and Bioengineering
Volume	71
Issue number	1
DOIs	https://doi.org/10.1002/(SICI)1097-0290(200024)71:1<44::AID-BIT7>3.0.CO;2-J
State	Published - Dec 2000
Externally published	Yes

Keywords

Directed sorting
MicroKan reactor
Parallel synthesis
Radiofrequency tagging
Solid-phase synthesis
Split and pool

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1002/(SICI)1097-0290(200024)71:1<44::AID-BIT7>3.0.CO;2-J

Cite this

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abstract = "A modular system for high-output solid-phase combinatorial synthesis has been designed and developed. The system employs three technological innovations to achieve its high efficiency and reliability: (1) application of microreactors as the reaction units in solid-phase synthesis; (2) use of radiofrequency tagging as the non-chemical tracking method; and (3) development of the directed sorting technology for split and pool synthesis. The system has been successfully applied in the synthesis of compound libraries of several hundred to several thousand compounds in multi- milligrams per compound quantity by many organizations. (C) 2000 John Wiley and Sons, Inc.",

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AU - Xiao, Xiao Yi

AU - Li, Rongshi

AU - Zhuang, Hui

AU - Ewing, Bill

AU - Karunaratne, K.

AU - Lillig, John

AU - Brown, Rick

AU - Nicolaou, K. C.

PY - 2000/12

Y1 - 2000/12

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AB - A modular system for high-output solid-phase combinatorial synthesis has been designed and developed. The system employs three technological innovations to achieve its high efficiency and reliability: (1) application of microreactors as the reaction units in solid-phase synthesis; (2) use of radiofrequency tagging as the non-chemical tracking method; and (3) development of the directed sorting technology for split and pool synthesis. The system has been successfully applied in the synthesis of compound libraries of several hundred to several thousand compounds in multi- milligrams per compound quantity by many organizations. (C) 2000 John Wiley and Sons, Inc.

KW - Directed sorting

KW - MicroKan reactor

KW - Parallel synthesis

KW - Radiofrequency tagging

KW - Solid-phase synthesis

KW - Split and pool

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Solid-phase combinatorial synthesis using MicroKan reactors, Rf tagging, and directed sorting

Abstract

Keywords

ASJC Scopus subject areas

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